この研究は、種々の細胞株(MCF10A、MCF7、Jurkat、NIH3T3)を用いて、その細胞増殖、細胞死、生存力、DNA合成速度に対する超低周波磁界(ELF-MF:60Hz、1mT:ばく露時間4または16時間)の影響を調べた。次に遺伝子発現に対する影響をELF-MF(60Hz、2mT:ばく露時間16時間)で調べた。その結果、細胞数、生存力、DNA合成速度について、JurkatおよびNIH3T3細胞では一定の変化は見られなかったが、 MCF10A およびMCF7細胞では有意な低下が見られた;細胞死に対するELF-MFの影響はどの細胞株でも見られなかった;遺伝子発現については、遺伝子PMAIP1の発現に変化が確認された、と報告している。
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The effects of exposure of 4 different cell lines to a 60 Hz magnetic field on cell growth and gene expression should be investigated.
MCF10 A cells were investigated 1, 2, 3 and 4 days after exposure, the remaining cell lines were investigated only 3 days after exposure.
A separate sham exposure group was used for each exposure condition. A positive control (gamma radiation, 4 and 6 Gy) was used for gene expression analysis. All experiments were repeated thrice.
for the positive control group, cells were exposed to 4 and 6 Gy of gamma radiation from a 137 Cs gamma ray source at a dose rate of 3.81 Gy/min
周波数 | 60 Hz |
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タイプ |
|
ばく露時間 | continuous for 4 hours |
Additional information | MCF10 A only |
ばく露の発生源/構造 |
|
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チャンバの詳細 | 60 mm culture dish |
ばく露装置の詳細 | one dish per condition was placed in an incubator equipped with the exposure system; after exposure, the cell dish was transferred into an incubator without exposure system until investigation; temperature was maintained constantly at 37 ± 0.3°C, humidity was maintained at ~95%, CO2 was 5% |
Sham exposure | A sham exposure was conducted. |
測定量 | 値 | 種別 | Method | Mass | 備考 |
---|---|---|---|---|---|
磁束密度 | 0.1 mT | - | - | - | - |
周波数 | 60 Hz |
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タイプ |
|
ばく露時間 | continuous for 4 hours |
Additional information | MCF10 A only |
ばく露の発生源/構造 |
|
---|---|
Sham exposure | A sham exposure was conducted. |
測定量 | 値 | 種別 | Method | Mass | 備考 |
---|---|---|---|---|---|
磁束密度 | 0.5 mT | - | - | - | - |
周波数 | 60 Hz |
---|---|
タイプ |
|
ばく露時間 | continuous for 16 hours |
Additional information | MCF7 only |
ばく露の発生源/構造 |
|
---|---|
Sham exposure | A sham exposure was conducted. |
測定量 | 値 | 種別 | Method | Mass | 備考 |
---|---|---|---|---|---|
磁束密度 | 2 mT | - | - | - | - |
Exposure to 0.1 mT and 0.5 mT magnetic fields did not show any effects.
When exposed to 1 mT, MCF10 A and MCF7 cells showed consistent and significant decreases in cell numbers, cell viability and cell proliferation compared to sham exposed cells, whereas Jurkat cells and NIH3T3 cells did not show consistent effects. No significant effects on cell death were found in any cell line.
In MCF7 cells exposed to 2 mT, cell numbers were significantly decreased and the cell cycle distribution was significantly altered compared to sham exposed cells . Apoptosis was not affected by the exposure. In gene expression analysis, PMAIP1-expression was significantly upregulated compared to sham exposed cells.
The authors conclude that exposure to a 60 Hz magnetic field might affect cell growth in some cell lines, possibly in association with a cell cycle delay and an increased expression of PMAIP1.
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