研究のタイプ: 医学／生物学の研究 (experimental study)

[超低周波電磁界はヒトがん細胞株の増殖およびミトコンドリア活性に影響を与える] med./bio.

Extremely low frequency electromagnetic fields affect proliferation and mitochondrial activity of human cancer cell lines

著者: Destefanis M, Viano M, Leo C, Gervino G, Ponzetto A, Silvagno F
掲載誌: Int J Radiat Biol 2015; 91 (12): 964-972
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この研究は、超低周波電磁界（ELF-EMF）がヒトがん細胞株（数種類）のミトコンドリア活性に影響を与え、最終的にがん細胞の増殖に影響を与えるか否かを調べた。その結果、ELF-EMFの長期ばく露により、数種類のがん細胞株の増殖が低下した；そのような影響には、ATPレベルを変化させずにミトコンドリア活性が上昇することが関連していた、と報告している。

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研究目的（著者による）

The effects of exposure of different human cancer cell lines to a 50 Hz magnetic field on cell proliferation and the mitochondrial activity should be investigated.

詳細情報

For each exposure duration and each treatment, a separate control group was used (remark EMF-Portal: no details were given on procedure for controls).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 3, 6 or 7 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • rectangular
ばく露時間 continuous for 3, 6 or 7 days
ばく露装置
ばく露の発生源／構造
チャンバの詳細 cell culture dishes between coils in a box inside an incubator; the box shielded the cells and exposure apparatus from the background magnetic field and was made of stainless steel and the shield was made of a 2 mm thick inner layer of mu-metal and an outer layer made of a special aluminium-free alloy
ばく露装置の詳細 setup was composed of two independent couples of coaxial coils made of 200 loops (formed of copper wire, 0.3 mm in diameter) in the shielding box, each loop being 2.5 cm in length (with a resulting loop density of 8000 loops per meter) and wound into a tightly packed plastic frame; the frame had a cylindrical shape with an outer radius of 8 cm and the distance between the two coaxial coil couples was 8 cm; the cell culture dish was placed in the central part between the coils; the outer coils were supplied with a DC current and provided a static magnetic field of 45 µT to simulate the geomagnetic field; the inner coils (7.5 cm radius) were connected to an AC current generator (signal with a duty cycle of 50%); inside the box, the temperature and humidity were homogenous to the rest of the incubator
Additional information the residual magnetic field inside the shielding box measured around 1-2 µT
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 12 µT effective value - - -
Additional parameter details

the point-to-point variation for the total magnetic field was up to ± 5% of the nominal value

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Proliferation was significantly decreased in all exposed cell lines compared to the control groups.
Mitochondrial activity was significantly increased in SKBR3 and HT29 cells directly after exposure for 3 or 7 days. However, the effects were reversible as further cultivation without exposure negated the effect. The ATP level and the gene expression in mitochondria were not changed significantly by exposure to the magnetic field.
Protein expression of phosphorylated ERK, p53 and cytochrome C was significantly reduced in mitochondria of exposed SKBR3 cells compared to the control groups after 3 and 7 days of exposure.
All other parameters did not show significant differences between exposure and control groups.
The authors conclude that exposure of different human cancer cell lines to a 50 Hz magnetic field might reduce cell proliferation, possibly mediated by the modulation of mitochondrial activity.

研究の種別:

研究助成

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