Effect of extremely low frequency magnetic fields on cell proliferation and gene expression med./bio.

Aim of study (acc. to author)

Background/further details

Endpoint

• molecular biosynthesis: gene expression
• cell viability/cell division/proliferation: cell growth

Exposure

• • 60 Hz
  • 50/60 Hz
  • magnetic field

Extended view Compact view

Methods Endpoint/measurement parameters/methodology

• molecular biosynthesis: gene expression (DNA microarray and semi-quantitative and quantitative real-time RT-PCR; only MCF7 with 2mT)
• cell function: cell cycle distribution (propidium iodide stain, flow cytometry; only MCF7 with 2 mT)
• cell viability/cell division/proliferation: cell numbers (trypan blue stain, hemocytometer; all cells), cell viability (MTT assay, spectrophotometry), cell death (propidium iodide stain, flow cytometry), cell proliferation (bromodeoxyuridine incorporation, immunohistochemistry, spectrophotometry) (all cells, only 1 mT), apoptosis (caspase 3/7 enzyme activity, spectrophotometry; only MCF7 with 2 mT)

Main outcome of study (acc. to author)

Study funded by

• Inha University, Incheon, Korea
• Korea Institute of Energy Technology and Planning (KETEP), Korea
• National Research Foundation (NRF) of Korea

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