この研究は、非腫瘍形成性ヒト細胞株（ヒト肺線維芽細胞WI-38およびヒト肺上皮細胞L132）において、60Hz磁界（MF）と電離放射線（IR）またはH2O2との組合せばく露がDNA損傷反応に影響するか否かを調べた。MF（0、1、2 mT）への6時間ばく露、IR（0、0.5、1、2 Gyのガンマ線）への1回ばく露後6時間培養、またはH2O2（0、0.1、0.5、1 mM）添加後に6時間培養のそれぞれ単独ばく露の他、組合せばく露として、MF＋IR（2 mT＋1 Gy）、MF＋H2O2（2 mT＋0.05または1 mM）の6時間ばく露を行い、リン酸化ヒストン2AX（γ-H2AX）発現およびγ-H2AXフォーカス生成（DNA2重鎖切断のマーカ）を調べた。その結果、磁界単独では、無ばく露対照に比べ、2 mTでγ-H2AX発現とγ-H2AXフォーカス生成の増加が見られたが、1 mTでは見られなかった；MF＋IRばく露の場合、IR単独に比べ、γ-H2AX発現とγ-H2AXフォーカス生成の増加が高まった；MF＋H2O2ばく露では、H2O2単独との差が無かった、と報告している。
The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →
To examine whether extremely low frequency magnetic fieldsalone and in combination with ionizing radiation or hydrogen peroxide (H2O2) induce DNA damage in human lung fibroblasts and human lung epithelial cells.
Cells were subjected to different treatments: 1.) control group, 2.) magnetic field exposure alone (1 mT or 2 mT), 3.) magnetic field exposure (2 mT) in the presence of H2O2 (fibroblasts: 0.05mM; epithelial cells: 1 mM) and 4.) magnetic field exposure (2 mT) and afterwards radiation with gamma rays (1 Gy). As positive controls, the cells were treated with different doses of gamma rays (single dose 0-2 Gy, dose rate of 5 Gy/min) or cultivated with different concentrations of H2O2 (0-1 mM) for 6 hours.
| 周波数 | 60 Hz |
|---|---|
| タイプ |
|
| ばく露時間 | continuous for 6 hours |
| Additional information | magnetic field + ionizing radiation |
| ばく露の発生源／構造 |
|
|---|---|
| Sham exposure | A sham exposure was conducted. |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 2 mT | - | - | - | - |
| 周波数 | 60 Hz |
|---|---|
| タイプ |
|
| ばく露時間 | continuous for 6 hours |
| Additional information | magnetic field + hydrogen peroxide |
| チャンバの詳細 | cells were cultivated and exposed in 60 mm cell culture dishes |
|---|---|
| ばく露装置の詳細 | temperature in exposure chamber was 37°C ± 0.3°C |
| Sham exposure | A sham exposure was conducted. |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 2 mT | - | - | - | - |
In both cell lines, exposure to a magnetic field alone with the intensity of 2 mT led to a significant increase in DNA double strand breaks compared to the control group, while a magnetic field with the intensity of 1 mT had no significant effect. The combination of magnetic field exposure and gamma radiation led to significant more DNA double strand breaks in both cell lines than one of the treatments alone, while no synergistic effect was found regarding the combination of magnetic field exposure and H2O2.
The authors conclude that extremely low frequency magnetic fields could induce DNA damage in human lung fibroblasts and human lung epithelial cells. Additionally, the data indicate that magnetic field exposure and gamma radiation could act synergistically.
このウェブサイトはクッキー（Cookies）を使って、最善のブラウジングエクスペリエンスを提供しています。あなたがこのウェブサイトを継続して使用することで、私たちがクッキーを使用することを許可することになります。
