この研究は、静磁界および超低周波磁界(ELF MF)へのばく露が細胞内酸化還元バランスに与える影響を調べた。その結果、9 mTの静磁界ばく露により、U937細胞での活性酸素種(ROS)の大きな増加およびグルタチオン(GSH)の細胞内レベル低下が、ばく露の2時間後に見られ、この変化はその後持続した;このため、細胞内酸化還元バランスは有意に変化した;また、ELF MFばく露も細胞内GSHに影響を与えることが示された;この影響の開始閾値は0.09 mTであった;また、ELF MFばく露は、U937細胞でのアポトーシスからの保護作用も示した;この保護作用をもたらす磁界の強度は、酸化還元バランスを変化させるELF MFの強度までに限られていたことから、MFの抗アポトーシス効果とMFによる細胞内酸化還元バランス変化との関連が示唆された、と報告している。
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This in vitro study was performed to investigate the effects of a static magnetic field and an extremely low frequency magnetic field on intracellular oxidation-reduction, and the eventual relationship between magnetic field exposure, oxidative alteration, and apoptosis in human cancer cells.
In a previous study (publication 300) the exposure of the same cell line to a static magnetic field showed a decrease in apoptosis by increasing magnetic flux density. Here, the influence of magnetic field exposure through intracellular oxidation-reduction on apoptosis should be examined.
ばく露の発生源/構造 |
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ばく露装置の詳細 | cell cultures exposed to magneic field inside the Faraday cage |
Sham exposure | A sham exposure was conducted. |
周波数 |
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タイプ |
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ばく露時間 | 2 h, 4 h, 72 h |
ばく露の発生源/構造 |
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ばく露装置の詳細 | magnets were placed under the culture petri dish |
Sham exposure | A sham exposure was conducted. |
測定量 | 値 | 種別 | Method | Mass | 備考 |
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磁束密度 | 0.6 mT | - | 計算値 | - | - |
The static magnetic field caused a strong increase of reactive oxygen species. After 2 hours of magnetic field exposure a significant decrease of intracellular glutathione levels was found and maintained thereafter, up to 72 hours.
The extremely low frequency magnetic field significantly decreased intracellular glutathione levels starting from a threshold at 0.09 mT.
Exposure to extremely low frequency magnetic field revealed an anti-apoptotic effect. The treshold for the anti-apoptotic effect corresponded with the treshold necessary to induce oxidation-reduction imbalance. Apoptosis was not affected by the exposure to extremely low frequency magnetic field in cells artificially deprived of glutathione.
The extremely low frequency magnetic field and the static magnetic field seem to exert a similar effect in terms of apoptosis, but they differ clearly in their thresholds: 0.6 mT for the static magnetic field and 0.09 mT for the extremely low frequency magnetic field.
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