Studientyp:
Medizinische/biologische Studie
(experimentelle Studie)
Induction of genomic instability, oxidative processes, and mitochondrial activity by 50Hz magnetic fields in human SH-SY5Y neuroblastoma cells
med./bio.
[Auslösung von genomischer Instabilität, oxidativen Prozessen und mitochondrialer Aktivität durch 50 Hz-Magnetfelder in menschlichen SH-SY5Y Neuroblastom-Zellen]
Von:
Luukkonen J, Liimatainen A, Juutilainen J, Naarala J
Veröffentlicht in: Mutation Research - Fundamental and Molecular Mechanism of Mutagenesis 2014; 760: 33-41
Es sollten die zellulären Veränderungen während der ersten 24 Stunden einer Exposition bei einem 50 Hz-Magnetfeld untersucht werden, und es sollte überprüft werden, ob die verursachten Veränderungen zu genomischer Instabilität bei den Abkömmlingen der exponiertenZellen führen könnten.
Hintergrund/weitere Details
In vorherigen Studien (Markkanen et al. 2008, Luukkonen et al. 2011) fanden die Autoren, dass eine Vorab-Exposition bei einem 50 Hz-Magnetfeld vor einer Menadion-Behandlung die Zell-Reaktion verändert und die Genotoxizität erhöht. Menadion ist eine Substanz, die freie Radikale produziert und die DNA schädigt. Die Zellen wurden für 24 Stunden bei einem Magnetfeldexponiert oder schein-exponiert. Anschließend wurden sie für drei Stunden mit oder ohne Menadion-Behandlung inkubiert (verschiedene Konzentrationen an Menadion wurden eingesetzt). Die Häufigkeit an Mikronuklei wurde 11 oder 18 Tage nach der Menadion-Behandlung bestimmt. Alle anderen Assays wurden direkt im Anschluss an die Magnetfeld-Exposition/Schein-Exposition, direkt nach der Menadion-Behandlung oder 8 oder 15 Tage nach der Menadion-Behandlung durchgeführt.
a pair of coils (34 x 46 cm) was placed in an incubator with 5 % CO2 and 37°C, distance between the coils 22 cm, coils generated a horizontal magnetic field, cells were positioned at the center of the coils for ensuring a uniform magnetic flux density
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Direction-Dependent Effects of Combined Static and ELF Magnetic Fields on Cell Proliferation and Superoxide Radical Production
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Modification of p21 level and cell cycle distribution by 50 Hz magnetic fields in human SH-SY5Y neuroblastoma cells
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