Exposure to 50 Hz electromagnetic field raises the levels of the anti-apoptotic protein BAG3 in melanoma cells med./bio.

[Exposition bei einem elektromagnetischem 50 Hz-Feld erhöht den Gehalt des anti-apoptotischen Proteins BAG3 in Melanom-Zellen]

Ziel der Studie (lt. Autor)

Hintergrund/weitere Details

Endpunkt

• Apoptose, Tumor-Wachstum, Protein-Expression von BAG3 und HSP70, zellulärer Stress

Exposition/Befeldung (teilweise nur auf Englisch)

• • 50/60 Hz
  • magnetisches Feld

Vollständige Ansicht Kompaktansicht

Methoden Endpunkt/Messparameter/Methodik

• molekulare Biosynthese: Protein-Expression von BAG3 und HSP70/HSP72 (in den Zellen und den herausgeschnittenen Tumoren; Western Blot); Gehalt reaktiver Sauerstoffspezies (als Stress-Marker; Durchflusszytometrie)
• Zellfunktionen: Messung der mitochondrialen Membran-Depolarisation (als Stress-Marker; Durchflusszytometrie)
• Zelllebensfähigkeit/Zellteilung/Zellproliferation: Apoptose (Propidiumiodid-Färbung; Durchflusszytometrie)
• Krebs: Tumor-Wachstum

Hauptergebnis der Studie (lt. Autor)

Studie gefördert durch

• Associazione Italiana per la Ricerca sul Cancro (AIRC; Italian Association for Cancer Research), Italy
• Ministero dell' Università e della Ricerca (MIUR (formerly MURST (Ministero dell' Università e della Ricerca Scientifica e Tecnologica); Ministry of University and Research), Italy
• Ministero della Salute (Ministry of Health), Italy

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