Induction of kinetochore-positive and kinetochore-negative micronuclei in CHO cells by ELF magnetic fields and/or X-rays med./bio.

[Induktion Kinetochor-positiver und Kinetochor-negativer Mikronuklei bei CHO-Zellen im extrem niederfrequenten Magnetfeld und/oder bei Röntgenstrahlung]

Ziel der Studie (lt. Autor)

Hintergrund/weitere Details

Endpunkt

• Genotoxizität/Mutation: Chromosomen-Veränderung (Mikronuklei-Bildung)

Exposition/Befeldung (teilweise nur auf Englisch)

• • 50/60 Hz
  • magnetisches Feld
  • auch andere Expositionen ohne EMF
• • Ko-Exposition

Vollständige Ansicht Kompaktansicht

Methoden Endpunkt/Messparameter/Methodik

• Genotoxizität/Mutation: Mikronuklei-Bildung, Mikronukleus-Anzahl in binukleären Zellen, Mikronukleus positiv/negativ (Zytokinese-Block-Mikronukleus-Assay, Fluoreszenzmikroskopie, Immunofluoreszenz-PI&FITC-Färbung mit Antikörpern gegen das Kinetochor)

Hauptergebnis der Studie (lt. Autor)

Studie gefördert durch

• Ministry of Education, Culture, Sports, Science and Technology, Japan
• Research for Future Program, Japan Society for the Promotion of Science (JSPS), Japan

Themenverwandte Artikel

• Yoon HE et al. (2014): Increased gamma-H2AX by exposure to a 60-Hz magnetic fields combined with ionizing radiation, but not hydrogen peroxide, in non-tumorigenic human cell lines
• Jin YB et al. (2014): Absence of DNA damage after 60-Hz electromagnetic field exposure combined with ionizing radiation, hydrogen peroxide, or c-Myc overexpression
• Li Y et al. (2014): Extra-low-frequency magnetic fields alter cancer cells through metabolic restriction
• Miyakoshi Y et al. (2012): Tempol suppresses micronuclei formation in astrocytes of newborn rats exposed to 50-Hz, 10-mT electromagnetic fields under bleomycin administration
• Jin YB et al. (2012): Effects on micronuclei formation of 60-Hz electromagnetic field exposure with ionizing radiation, hydrogen peroxide, or c-Myc overexpression
• Cho YH et al. (2007): Effects of extremely low-frequency electromagnetic fields on delayed chromosomal instability induced by bleomycin in normal human fibroblast cells
• Mairs RJ et al. (2007): Microsatellite analysis for determination of the mutagenicity of extremely low-frequency electromagnetic fields and ionising radiation in vitro
• Udroiu I et al. (2006): Clastogenicity and aneuploidy in newborn and adult mice exposed to 50 Hz magnetic fields
• Fatigoni C et al. (2005): Genotoxic effects of extremely low frequency (ELF) magnetic fields (MF) evaluated by the Tradescantia-micronucleus assay
• Scarfi MR et al. (2005): Evaluation of genotoxic effects in human fibroblasts after intermittent exposure to 50 Hz electromagnetic fields: a confirmatory study
• Winker R et al. (2005): Chromosomal damage in human diploid fibroblasts by intermittent exposure to extremely low-frequency electromagnetic fields
• Stronati L et al. (2004): Absence of genotoxicity in human blood cells exposed to 50 Hz magnetic fields as assessed by comet assay, chromosome aberration, micronucleus, and sister chromatid exchange analyses
• Testa A et al. (2004): Evaluation of genotoxic effect of low level 50 Hz magnetic fields on human blood cells using different cytogenetic assays
• Verheyen GR et al. (2003): Effect of coexposure to 50 Hz magnetic fields and an aneugen on human lymphocytes, determined by the cytokinesis block micronucleus assay
• Cho YH et al. (2003): The effect of extremely low frequency electromagnetic fields (ELF-EMF) on the frequency of micronuclei and sister chromatid exchange in human lymphocytes induced by benzo(a)pyrene
• Pasquini R et al. (2003): Micronucleus induction in cells co-exposed in vitro to 50 Hz magnetic field and benzene, 1,4-benzenediol (hydroquinone) or 1,2,4-benzenetriol
• Zeni O et al. (2001): Combined exposure to extremely low frequency (ELF) magnetic fields and chemical mutagens: Lack of genotoxic effects in human lymphocytes
• Simko M et al. (2001): Micronucleus induction in Syrian hamster embryo cells following exposure to 50 Hz magnetic fields, benzo(a)pyrene, and TPA in vitro
• Abramsson-Zetterberg L et al. (2001): Extended exposure of adult and fetal mice to 50 Hz magnetic field does not increase the incidence of micronuclei in erythrocytes
• Simko M et al. (1999): Absence of synergistic effects on micronucleus formation after exposure to electromagnetic fields and asbestos fibers in vitro
• Scarfi MR et al. (1999): Micronucleus frequency and cell proliferation in human lymphocytes exposed to 50 Hz sinusoidal magnetic fields
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• Simko M et al. (1998): Effects of 50 Hz EMF exposure on micronucleus formation and apoptosis in transformed and nontransformed human cell lines
• Scarfi MR et al. (1997): 50-Hz, 1-mT sinusoidal magnetic fields do not affect micronucleus frequency and cell proliferation in human lymphocytes from normal and Turner's syndrome subjects
• Scarfi MR et al. (1997): Cytogenetic effects induced by extremely low frequency pulsed magnetic fields in lymphocytes from Turner`s syndrome subjects
• Lagroye I et al. (1997): The effect of 50 Hz electromagnetic fields on the formation of micronuclei in rodent cell lines exposed to gamma radiation
• Scarfi MR et al. (1994): Lack of chromosomal aberration and micronucleus induction in human lymphocytes exposed to pulsed magnetic fields
• Scarfi MR et al. (1993): 50 Hz AC sinusoidal electric fields do not exert genotoxic effects (micronucleus formation) in human lymphocytes
• Scarfi MR et al. (1991): Spontaneous and mitomycin-C-induced micronuclei in human lymphocytes exposed to extremely low frequency pulsed magnetic fields