この研究は、スイスアルビノマウスに無線周波放射(RFR:900 MHz、1日のばく露時間は4時間または8時間、35日間継続)を与え、1群はばく露終了直後、他群はばく露終了からさらに35日間の回復期間を経た後に、精巣生殖細胞の変化を調べた。その結果、RFRばく露群にミトコンドリア膜電位の低下が見られた;生殖細胞の損傷指標上昇、精子頭部異常、細胞周期の異常なども見られた;しかしこれらの変化は、ばく露後の35日間で回復が見られた、と報告している。
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The effects of exposure of mice to a 900 MHz electromagnetic field (GSM) on the cell cycle and DNA damage in sperms and testes histology should be investigated.
Mice were divided into 5 groups (n=15 each): exposure to the electromagnetic field for 1) 4 h/day for 35 days, 2) 4 h/day for 35 days and subsequent unexposed maintenance for 35 days, 3) 8 h/day for 35 days, 4) 8 h/day for 35 days and subsequent unexposed maintenance for 35 days, 5) control group. Groups 2 and 4 were used to analyze post-exposure recovery effects.
ばく露 | パラメータ |
---|---|
ばく露1:
902.4 MHz
Modulation type:
pulsed
ばく露時間:
4 h/day for 35 days
|
|
ばく露2:
902.4 MHz
Modulation type:
pulsed
ばく露時間:
8 h/day for 35 days
|
周波数 | 902.4 MHz |
---|---|
タイプ |
|
ばく露時間 | 4 h/day for 35 days |
Modulation type | pulsed |
---|---|
Repetition frequency | 217 Hz |
Additional information |
similar to GSM |
ばく露の発生源/構造 |
|
---|---|
チャンバの詳細 | chromium-nickel metal container |
ばく露装置の詳細 | animals could move freely during exposure; minimum and maximum distance of animals from antenna was 4.5 cm and 22.5 cm |
周波数 | 902.4 MHz |
---|---|
タイプ |
|
ばく露時間 | 8 h/day for 35 days |
Modulation type | pulsed |
---|---|
Repetition frequency | 217 Hz |
Additional information |
similar to GSM |
ばく露の発生源/構造 |
|
---|
Exposure to the electromagnetic field of groups 3 and 4 caused a significantly reduced mitochondrial membrane potential compared to the control group, though potential was higher after recovery (group 4). Depolarization of the mitochondrial membrane leads to impaired cellular redox homeostasis causing oxidative stress.
The number of abnormal sperm cells with head defects was significantly increased in exposure groups 1, 3 and 4 compared to the control group.
Analysis of germ cell subpopulations in testis showed a significant increase of spermatogonia and a significant decrease in spermatids in groups 3 and 4 compared to the control group. This indicated cell cycle arrest in the pre-meiotic stage of spermatogenesis, which resulted in loss of post-meiotic germ cells.
Histological investigation showed significantly reduced epithelial area and significantly increased loss of tissue in seminiferous tubules in group 3 compared to the control group, but not in group 4. DNA damage was significantly increased in exposure groups 1, 3 and 4 compared to the control group, with most damage found in groups 1 and 3.
The authors conclude that exposure of mice to a 900 MHz electromagnetic field (GSM) might induce oxidative stress, which causes DNA damage that leads to alterations of the cell cycle and to testicular damage. However, these effects might be reversible.
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