研究のタイプ: 医学／生物学の研究 (experimental study)

[マイクロ波放射のばく露を受けたラットの精巣組織における酸化ストレスパラメータおよびDNA断片化に対するメラトニンの影響] med./bio.

The Effects of Melatonin on Oxidative Stress Parameters and DNA Fragmentation in Testicular Tissue of Rats Exposed to Microwave Radiation

著者: Sokolovic D, Djordjevic B, Kocic G, Stoimenov TJ, Stanojkovic Z, Sokolovic DM, Veljkovic A, Ristic G, Despotovic M, Milisavljevic D, Jankovic R, Binic I
掲載誌: Adv Clin Exp Med 2015; 24 (3): 429-436
RISフォーマットで参考文献をダウンロード

この研究は、マイクロ波ばく露（4時間／日）を受けたラットの精巣組織における酸化ストレスパラメータおよびDNA断片化に対するメラトニン処置の影響を評価した。成獣ウィスターラットを4群に分けた：生食投与群、メラトニン投与群、マイクロ波ばく露群、マイクロ波ばく露＋メラトニン投与群。処置後20、40、60日目に屠殺して、精巣組織を検査した。その結果、メラトニンの抗酸化作用により、マイクロ波ばく露を受けた精巣での酸化ストレスの強度低下およびDNA断片化の減少が見られた、と報告している。

The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →

研究目的（著者による）

The effects of exposure of rats to a 900 MHz electromagnetic field (GSM) on oxidative stress in the testes should be investigated and if an administration of melatonin prevents these effects.

詳細情報

Rats were divided into the following groups (n=21 each): 1) GSM exposure, 2) melatonin group (intraperitoneal injection of 2 mg/kg melatonin), 3) co-exposure (melatonin injection and GSM exposure), 4) control group (injection with saline).
After 20, 40 and 60 days, 7 rats from each group were sacrificed and analyzed, respectively.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: CW
ばく露時間: continuous for 4 hours/day for 20, 40 or 60 days

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • near field
ばく露時間 continuous for 4 hours/day for 20, 40 or 60 days
Additional information GSM signal
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
  • セルラ－電話
  • Nokia 3110
チャンバの詳細 plastic cage without any metallic fittings
ばく露装置の詳細 8 rats per cage (remark EMF-Portal: but 7 rats per cage were housed and sacrificed) were able to move freely; test phone was connected to a computer with an appropriate software module by a communication test set PC development kit
パラメータ
測定量 値 種別 Method Mass 備考
電界強度 9.88 V/m minimum 測定値 - -
電界強度 18.36 V/m maximum 測定値 - -
磁束密度 4.68 µT minimum 測定値 - -
磁束密度 8.69 µT maximum 測定値 - -
SAR 0.043 W/kg minimum 推定値 whole body -
SAR 0.135 W/kg maximum 推定値 whole body -

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

After 20 days, only the lipid peroxidation and the enzyme activity of the alkaline DNase were significantly increased and the enzyme activity of the catalase was significantly reduced in the testes of GSM exposed mice (group 1) compared to the control group (group 4). After 40 and 60 days, the lipid peroxidation, protein oxidation and enzyme activities of xanthine oxidase, alkaline and acid DNase were significantly increased and the enzyme activity of the catalase was significantly reduced in GSM exposed mice compared to the control group.
Co-exposure to the GSM field and melatonin (group 3) significantly reduced lipid peroxidation, xanthine oxidase and acid DNAse enzyme activities compared to the GSM exposure group.
Mice injected with melatonin only (group 2) showed a significantly reduced acid DNAse enzyme activity after 20-60 days compared to the control group.
The authors conclude that exposure of rats to a 900 MHz electromagnetic field (GSM) could induce oxidative stress in the testes and that melatonin might attenuate the effect.

研究の種別:

研究助成

関連論文