Radiofrequency radiation (900 MHz)-induced DNA damage and cell cycle arrest in testicular germ cells in swiss albino mice med./bio.

Aim of study (acc. to author)

Background/further details

Endpoint

• effects on the reproductive system: cell cycle and DNA damage in sperms

Exposure

• • 900 MHz
  • RF
• • mobile communications
  • mobile phone
  • GSM

Extended view Compact view

Methods Endpoint/measurement parameters/methodology

• genotoxicity/mutation: DNA damage in germ cells (strand breaks, alkaline comet assay)
• effects on the reproductive system: sperm count (total and abnormal sperm; hemocytometer) and head abnormalities (amorphous, beaked, hooked, hookless, pin headed, double headed, banana shaped, giant and dwarf heads; Eosin Y stain; light microscopy) in epididymis, tissue index (weight of testis, epididymis and vas deferens in relation to body weight), testes histology (amount of seminiferous tubules with loss of tissue per mouse, epithelium area per seminiferous tubule; hematoxylin-eosin stain, light microscopy), germ cell maturation/cell cycle in testes (groups 3-5, ratios of germ cell subpopulations spermatids, spermatogonia and secondary spermatocytes and primary spermatocytes; propidium iodide stain, flow cytometry), mitochondrial membrane potential in testicular cells (groups 3-5, JC-1 stain, commercial kit)

Main outcome of study (acc. to author)

Study funded by

• Department of Science and Technology (DST), India
• University Grants Commission (UGC), India

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