この研究は、マウス実験で超低周波電磁界(ELF-EMF:50Hz、8mT)へのばく露(28日間)による影響に対するハスの莢抽出成分プロシアニジン(LSPCs)の保護効果とそのメカニズムを検討した。その結果、LSPCsがマウスの臓器指数を上昇させ、ELF-EMFばく露により損傷された造血機能およびサイトカインレベルを見かけ上の通常レベルにまで回復させることが示されたとして、そのメカニズムについて議論している。
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The effects of exposure of mice to a 50 Hz magnetic field on the immune functions of the spleen and the protective effect of lotus seedpod procyanidins should be investigated.
Several studies showed that procyanidins have several biological functions, among others, they might enhance the function of the immune system.
Mice were divided into 5 groups (n=10 each): 1) exposure to the magnetic field and saline solution administration, 2) exposure to the magnetic field and administration of 30 mg/kg lotus seedpod procyanidins (LSPC) extract, 3) exposure to the magnetic field and administration of 60 mg/kg LSPC extract, 4) exposure to the magnetic field and administration of 90 mg/kg LSPC extract, 5) control group.
ばく露 | パラメータ |
---|---|
ばく露1:
50 Hz
ばく露時間:
4 h/day for 28 days
|
|
周波数 | 50 Hz |
---|---|
タイプ |
|
ばく露時間 | 4 h/day for 28 days |
ばく露の発生源/構造 |
|
---|---|
Sham exposure | A sham exposure was conducted. |
測定量 | 値 | 種別 | Method | Mass | 備考 |
---|---|---|---|---|---|
磁束密度 | 8 mT | - | - | - | - |
Exposure to the magnetic field (group 1) resulted in a significant reduction of relative weights of thymus, spleen and kidney compared to the control group. Mice with an additional administration of 90 mg/kg LSPCs (group 4), however, showed a significant increase of the organ weights compared to group 1.
The amounts of leukocytes, lymphocytes and hemoglobin were significantly reduced in group 1 compared to the control group, while they were significantly increased in group 4 compared to group 1.
The protein expressions of INF-gamma, IL-2, IL-6 and IL-10 were significantly reduced and the expression of TNF-alpha was significantly increased in group 1 compared to the control group. These effects were significantly reduced in groups 3 and 4.
The DNA content was significantly reduced in group 1 compared to the control group and it was significantly increased in group 4 compared to group 1.
The number of early and late apoptotic splenocytes was significantly reduced in group 1 compared to the control group and it was significantly increased in groups 3 and 4 compared to group 1.
In cell cycle analysis, group 1 had a significant larger G0 phase/G1 phase fraction and smaller S phase fraction compared to the control group. This effect was reduced by an additional LSPCs treatment (groups 2-4).
The magnetic field (group 1) significantly reduced Bcl-2 and Bcl-xl protein expression and significantly increased Bax, Caspase-3 and -9 levels. These effects were attenuated by LSPCs treatment and in group 4 there were significant differences in all expression levels compared to group 1.
The authors conclude that exposure of mice to a 50 Hz magnetic field might impair splenocytes via activation of Bax, caspases-3 and -9, and that lotus seedpod procyanidins could have a protective effect.
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