研究のタイプ: 医学／生物学の研究 (experimental study)

[弱い50Hz磁界にばく露した神経芽細胞腫および神経膠腫の細胞株での小核およびスーパーオキシド産生の誘導] med./bio.

Induction of micronuclei and superoxide production in neuroblastoma and glioma cell lines exposed to weak 50 Hz magnetic fields

著者: Kesari KK, Juutilainen J, Luukkonen J, Naarala J
掲載誌: J R Soc Interface 2016; 13 (114): 13-
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この研究は、神経芽細胞腫細胞株SH-SY5Yおよび神経膠腫細胞株C6への10および30µTの50Hz磁界（MFs）の24時間ばく露および共因子メナジオン（MQ）の影響を調べた。その結果、両方の細胞株に、それぞれ異なるMFsの影響が見られた；SH-SY5Yでは30µTで小核が有意に増加し、MQの最大投与量において変化が最大であった；C6では、細胞質ゾルおよびミトコンドリアのスーパーオキシドレベル上昇が見られ、この影響にMQは無関係であり、また10µTでも大きな変化が見られた、と報告している。

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研究目的（著者による）

The effects of co-exposure of human neuroblastoma and rat glioma cells to a 50 Hz magnetic field and menadione on DNA damage and oxidative stress should be investigated.

詳細情報

The administration of menadione for co-exposure was based on previous findings indicating that exposure to magnetic fields alters cellular responses to menadione (Markkanen et al. 2008, Luukkonen et al. 2011). Menadione leads to the prodution of superoxide anions and DNA damage.
Neuroblastoma cells were treated with 0, 1, 5, 10 and 20 µM and glioma cells were treated with 0, 1, 5, 10, 15, 20 and 50 µM menadione and were either not exposed to the magnetic field (control groups), sham exposed or exposed to a 10 or 30 µT magnetic field.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 24 hours
ばく露2: 50 Hz
ばく露時間: continuous for 24 hours

General information

menadione was administered after exposure to the magnetic field and cells were incubated for 3 hours afterwards

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 24 hours
ばく露装置
ばく露の発生源／構造
チャンバの詳細 cell culture dishes in coil system in incubator (temperature-controlled and 5% CO2)
ばく露装置の詳細 horizontal magnetic field was generated by a pair of coils (34 cm x 46 cm) in a Helmholtz-type configuration (22 cm distance between the coils); cell culture dishes were placed at the center of the coil system, where the magnetic field was homogeneous
Sham exposure A sham exposure was conducted.
Additional information control cells were incubated in an identical incubator as exposed and sham exposed cells
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 10 µT - 測定値 - -
Additional parameter details

there were varying background AC magnetic field levels inside the incubators due to the on-off cycle of the heating system; there were also minor differences between the four locations used for cell cultures; the maximum (short peak value in the location with the highest field when heating was switched on by the thermostat) was 2.35 µT in the control incubator and 1.83 µT in the exposure incubator; the minimum values (heater off) were about 0.01 µT in both incubators; the predominant direction of the background AC magnetic field was horizontal, from front to back of the incubator (perpendicular to the experimental 50 Hz field); the static magnetic field in the incubators was almost vertical with 29.5 µT in the control incubator and 27.5 µT in the exposure incubator

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 24 hours
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 30 µT - 測定値 - -

Reference articles

  • Luukkonen J et al. (2014): [ヒト神経芽腫細胞SH-SY5Yにおけるゲノム不安定性、酸化プロセス、ミトコンドリア活性の50Hz磁界による誘導]
  • Markkanen A et al. (2008): [50Hz磁界への事前ばく露を受けたマウスL929細胞ではメナジオン誘導性DNA損傷応答が変化する]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Sham exposure did not show any significant effects in any parameter compared to the control groups in both cell lines.
Micronuclei formation was significantly increased in neuroblastoma cells exposed to the 30 µT magnetic field compared to the control group. It was most pronounced in cells co-exposed to the highest concentration of menadione (20 µM) and a significant interaction of menadione and the magnetic field exposure was detected. That means, that the effect of the magnetic field was dependent on the presence and amount of menadione. In C6 cells, the magnetic field had no significant effect on micronuclei formation compared to the control groups.
Cytosolic and mitochondrial superoxide production were significantly increased only in C6 cells exposed to 10 µT or 30 µT magnetic fields compared to the control groups. No interactions of the magnetic field and menadione were observed.
Cell viability was not affected significantly by the magnetic field or co-exposure to the magnetic field and menadione.
The authors conclude that exposure to a 50 Hz magnetic field might provoke DNA damage in human neuroblastoma cells and oxidative stress in rat glioma cells. In the genotoxic effects, the magnetic field enhanced the effcts of menadione in a co-exposure, while the effects on oxidative stress were independent of co-exposure.

研究の種別:

研究助成

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