研究のタイプ: 医学／生物学の研究 (experimental study)

[Tempolはブレオマイシン投与下で50 Hz、10 mTの電磁界にばく露した新生仔ラット星状細胞の小核形成を抑制する] med./bio.

Tempol suppresses micronuclei formation in astrocytes of newborn rats exposed to 50-Hz, 10-mT electromagnetic fields under bleomycin administration

著者: Miyakoshi Y, Kajihara C, Shimizu H, Yanagisawa H
掲載誌: Mutat Res Genet Toxicol Environ Mutagen 2012; 747 (1): 138-141
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この研究は、50 Hz, 10 mTの磁界とブレオマイシン（BLM）の共ばく露による突然変異源的影響を新生仔ラットの星状細胞における小核アッセイにより調べた。またこの突然変異源的影響に活性酸素種が関連するか否かを、スーパーオキサイドラジカルのスカベンジャーである薬剤Tempolを用いて調べた。3日齢SD雄ラットを用いて、前者の実験は6群（n = 6）：磁界ばく露72時間（有：無）×BLM（無し：5 mg/kg：10 mg/kg）［ばく露開始前1回投与］、後者の実験は4群（n = 6）：全群ともBLM10 mg/kg投与後、磁界ばく露（有：無）×Tempol 200 μmol/kg（有：無）［BLM投与前に1回目、以降12時間おきに72時間目まで全7回投与］で行った。その結果、星状細胞の小核頻度は、BLM10 mg/kg+磁界ばく露群の方がBLM10 mg/kg＋擬似ばく露群より1.6倍高かった(p < 0.05)；BLM10 mg/kgと磁界の72時間共ばく露においてTompol処置を加えた群の小核頻度は、対照群（生食液処置）の40%であった(p < 0.01)、という所見を報告している。

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研究目的（著者による）

To study the mutagenic effects of co-exposure to 50 Hz magnetic fields and bleomycin. Additionally, it was investigated whether the mutagenic effects of magnetic fields were related to reactive oxygen species by using tempol (4-hydroxy-2,2,6,6-tetramethyl piperidine-1-oxyl), a superoxide radical scavenger.

詳細情報

Three-day-old male rats were sham exposed or co-exposed to 50 Hz magnetic fields and bleomycin (5 or 10 mg/kg body weight) in six groups (n=6 rats each): 1) magnetic field exposure, 2) sham exposure, 3) bleomycin (5 mg) + magnetic field co-exposure, 4) bleomycin (5 mg) + sham exposure, 5) bleomycin (10 mg) + magnetic field co-exposure, 6) bleomycin (10 mg) + sham exposure.
Additionally, four groups of rats (n=4 rats each) were treated with tempol: 7) bleomycin (10 mg/kg) + magnetic field exposure + tempol (200 µmol/kg body weight), 8) bleomycin + sham exposure + tempol, 9) bleomycin + magnetic field exposure + physiological saline solution, 10) bleomycin + sham exposure + physiological saline solution.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 72 h

General information

Rats were divided into 10 groups: 1) magnetic field exposure 2) sham exposure 3) bleomycin (5 mg/kg body weight) + magnetic field co-exposure 4) bleomycin (5 mg) + sham exposure 5) bleomycin (10 mg) + magnetic field co-exposure 6) bleomycin (10 mg) + sham exposure 7) bleomycin (10 mg/kg body weight) + magnetic field exposure + tempol (200 µmol/kg body weight) 8) bleomycin + sham exposure + tempol 9) bleomycin + magnetic field exposure + physiological saline solution 10) bleomycin + sham exposure + physiological saline solution

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 72 h
ばく露装置
ばく露の発生源／構造
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 10 mT - - - -

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

The number of micronucleated astrocytes in the "bleomycin (10 mg) + magnetic field exposure group" (group 5) was 1.6 times higher than that in the appropriate sham exposure group (group 6).
The number of micronuclei in astrocytes of group 7 (co-exposure to magnetic field + bleomycin + tempol) was 40% of that in rats administered with the same dose of bleomycin and physiological saline (group 9) and similar to those of the two sham exposure groups (groups 8 and 10).
The data suggest that the mechanism responsible for the elevated frequency of micronuclei in astrocytes of rats co-exposed to bleomycin and magnetic fields is related to reactive oxygen species.

研究の種別:

研究助成

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