研究のタイプ: 医学／生物学の研究 (experimental study)

[50 Hz磁界とベンゼン、ヒドロキノンなどとの共ばく露された細胞の微小核誘導] med./bio.

Micronucleus induction in cells co-exposed in vitro to 50 Hz magnetic field and benzene, 1,4-benzenediol (hydroquinone) or 1,2,4-benzenetriol

著者: Pasquini R, Villarini M, Scassellati Sforzolini G, Fatigoni C, Moretti M
掲載誌: Toxicol In Vitro 2003; 17 (5-6): 581-586
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この研究は、超低周波磁界（ELF MF）ばく露を受けたジャーカット細胞における遺伝毒性および/または共遺伝毒性の有無を、細胞質分裂ブロック小核（MN）法により測定した。磁束密度5 mTの50 Hz磁界（MF）を、単独で、またはベンゼン、1,4-ベンゼンジオール、または1,2,4-ベンゼントリオールと組み合わせて、さまざまな時間（1〜24時間）ばく露した。その結果、24時間のばく露の場合、MFばく露培養細胞における小核頻度は、擬似ばく露（対照）培養細胞のそれよりも1.9倍高かった；ベンゼンのばく露は細胞遺伝学的活性を示さなかった；1,4-ベンゼンジオールまたは1,2,4-ベンゼントリオールのばく露は、無処理に比べ、MNの数に有意な影響を与えた；さらに、ELF-MFの共ばく露を行なっても、ベンゼン、1,4-ベンゼンジオール、または1,2,4-ベンゼントリオールによって誘発される小核頻度に影響はなかった、と報告している。

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研究目的（著者による）

The genotoxic effects of co-exposure of Jurkat cells to a 50 Hz magnetic field and benzene and its metabolites should be investigated.

詳細情報

Benzene is known to cause leukemia. However, it is unlikely to be the ultimate carcinogen and therefore its metabolites hydroquinone and 1,2,4-benzenetriol were investigated as well.
Cells were divided into the following groups: 1) exposure to the magnetic field, 2) exposure to benzene, 3) exposure to hydroquinone, 4) exposure 1,2,4-benzenetriol, 5-7) co-exposure to the magnetic field and benzene, hydroquinone or 1,2,4-benzenetriol, respectively. For each group, a respective sham exposure group was used (remark EMF-Portal: a control group is mentioned as well but no details are given and no results are stated for it). Each group was investigated after 1 and 24 hours of exposure, respectively.
Results were obtained in three independent experiments. Positive controls and negative controls were conducted.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 1 hour
ばく露2: 50 Hz
ばく露時間: continuous for 24 hours

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 1 hour
ばく露装置
ばく露の発生源／構造
チャンバの詳細 dry CO2-incubator (37°C, 5% CO2)
ばく露装置の詳細 cells in test tubes (16 mm diameter and 100 mm length) were placed in the center of the induction coils (1000 turns of copper wire (0.66 mm diameter) around a scaffold of non-ferromagnetic matter, resulting in an inside diameter of 22 mm, an outside diameter of 38 mm, and a length of 60 mm); each induction coil was enclosed in a mantle of ferromagnetic steel to prevent EMF interference with other coils; current through coils was 0.25 A; magnetic field was parallel to the axis of the test tube and homogeneous; temperature during experiments was (37°C ± 0.3°C)
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 5 mT - 測定値および計算値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 24 hours
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 5 mT - 測定値および計算値 - -

Reference articles

  • Antonopoulos A et al. (1995): [In vitroでのヒトリンパ球に対する50 Hz電磁界の細胞影響]
  • Rosenthal M et al. (1989): [化学的な突然変異原での前処置または未処置のヒト末梢リンパ球の増殖と染色体変化に対する50 Hz電磁界の影響]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

After 24 hours, cells exposed to the magnetic field (group 1) or to benzene (group 2) alone showed significantly more DNA damages than their respective sham exposure groups.
Exposure to hydroquinone (group 3) or 1,2,4-benzenetriol (group 4) resulted in significantly increased DNA damage after 1 and 24 hours compared to their sham exposure groups. Co-exposure to the magnetic field did not influence the effects of benzene or its metabolites (groups 5-7).
The authors conclude the benzene metabolites hydroquinone and 1,2,4-benzenetriol as well as the 50 Hz magnetic field might have a genotoxic effect on Jurkat cells.

研究の種別:

研究助成

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