この研究は、ヒト好中球細胞株（HL-60、PLB-985）のカルシウムシグナル伝達に対する低周波電磁界（LF EMFs：Immunent波形または正弦波；磁束密度5μT、300μT、500μT）の影響の可能性を調べた。好中性顆粒球では、受容体に結合したGタンパク質の活性化により､特定のカルシウムチャネルを介したカルシウム流出または流入が生じる。この現象へのLF EMFsの関与を実験で調べた。その結果、走化性刺激時のカルシウム動員は、30分間の短期ばく露または長期ばく露により変化しなかった；波形や磁束密度を変えても結果は変わらなかった､と報告している。
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The adverse effects of exposure of human neutrophil granulocyte-like cells to a 50 Hz magnetic field or to a combination of several extremely low frequency and intermediate frequency fields on calcium signal pathways should be investigated.
In two different cell lines, HL-60 and PLB-985 cells, a differentiation into neutrophil-like cells was induced by the addition of DMSO. The combination of different low-frequency and intermediate frequency fields was called "Immunent field".
Both cell lines were exposed to two different types of fields (50 Hz and Immunent field) and 4 different magnetic flux densities (2,5 mT, 500 µT, 300 µT, and 5 µT) for 3 minutes, 30 minutes, 4 days or 5 days.
| ばく露 | パラメータ |
|---|---|
|
ばく露1:
50 Hz
ばく露時間:
continuous for 30 minutes
|
|
|
ばく露2:
50 Hz–2.6 kHz
ばく露時間:
continuous for 30 minutes
Immunent field
|
|
|
ばく露3:
50 Hz
ばく露時間:
continuous for 30 minutes, 4 days or 5 days
|
|
|
ばく露4:
50 Hz–2.6 kHz
ばく露時間:
continuous for 30 minutes, 4 days or 5 days
Immunent field
|
|
|
ばく露5:
50 Hz
ばく露時間:
intermittent for a total of up to 3 minutes
for flow cytometry
|
background alternating current magnetic flux densities at the place of exposure and sham exposure were less than 0.2 µT and ambient direct current static magnetic fields were 10 µT (except for exposure 5)
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| 波形 |
|
| ばく露時間 | continuous for 30 minutes |
| ばく露の発生源／構造 | |
|---|---|
| チャンバの詳細 | cell culture flasks |
| ばく露装置の詳細 | coil was placed inside a standard cell culture incubator with 37°C ± 0.2°C and 5% CO2; cell culture flasks were placed in the central region of the coils, perpendicular to vertical magnetic field lines; copper wire coil was wound on a double co-axial cylinder with an inner (65 turns; Ø 184 mm) and outer cylinder (2 times 8 turns; Ø 400 mm) made of polymethyl methacrylate; inhomogeneity within exposure area was < 0.4% |
| Sham exposure | A sham exposure was conducted. |
| Additional information | two identical cell culture incubators with identical exposure coil inside were used and randomly assigned to be active or sham for each experiment |
| 周波数 | 50 Hz–2.6 kHz |
|---|---|
| タイプ |
|
| ばく露時間 | continuous for 30 minutes |
| Additional information | Immunent field |
| Additional information | combination of four block waves at frequencies of 320, 730, 880 and 2600 Hz |
| ばく露の発生源／構造 |
|
|---|---|
| Sham exposure | A sham exposure was conducted. |
| ばく露の発生源／構造 |
|
|---|---|
| Sham exposure | A sham exposure was conducted. |
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| 波形 |
|
| ばく露時間 | intermittent for a total of up to 3 minutes |
| Additional information | for flow cytometry |
| ばく露の発生源／構造 | |
|---|---|
| チャンバの詳細 | test tubes |
| ばく露装置の詳細 | custom-made exposure system fitting around the test tube inside the flow cytometer; the circular coil consisted of 0.5 mm tightly wound copper wire around a polyether ether ketone body with an inner diameter of 38 mm (100 turns); the direction of the field was parallel to the height of the test tube |
| Sham exposure | A sham exposure was conducted. |
No significant differences could be found between the exposure groups and their corresponding sham exposure groups.
The authors conclude that exposure of human neutrophil granulocyte-like cells to a 50 Hz magnetic field or to a combination of several extremely low frequency and intermediate frequency fields has no effect on calcium signal pathways.
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