この研究は、ウィスター雄ラット(6-8週齢:n=36)を用いて、脳の異なる領域におけるチオバルビツール酸反応物質(TBARS)、総抗酸化能(TA)、グルタチオンSトランスフェラーゼ(GST)のレベルに対する900MHz無線周波電磁界(RF-EMR)の影響を調べた。ラットは12匹ずつの3群(ケージ対照群、擬似ばく露群、ばく露群)とし、ばく露群は900MHzRF-EMR(GSM携帯電話のサイレントモードを使用:ピーク電力密度146.60 µW/cm2、1日1時間)に4週間ばく露した。実験29日目に行動学的分析を行い、その後各群6匹について、扁桃、海馬、前頭葉、小脳の組織切片を検査した。その結果、ばく露群で行動学的タスクの成績の変化が見られた;全ての部位でTBARSレベル上昇が見られた;海馬のみでGSTレベルが低下した、などを報告している。
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The effects of a whole body exposure of adolescent rats to a 900 MHz electromagnetic GSM field on oxidative stress and the antioxidative system in discrete brain sections should be investigated.
36 rats were equally separated into the three following groups (n=12 in each group): 1) cage control, 2) sham exposure and 3) exposure. Subsequent to exposure (day 29), behavioral analysis was conducted (not part of the present paper, published here: Narayanan et al. 2013) and afterwards, six animals of each group were sacrificed and examined.
周波数 | 890–915 MHz |
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タイプ |
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ばく露時間 | intermittent for 1 h/day for 28 days |
Modulation type | pulsed |
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Repetition frequency | 217 Hz |
ばく露の発生源/構造 |
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チャンバの詳細 | polypropylene cages (3 animals per cage) measuring 41 cm Œ 28 cm Œ 14 cm, temperature 23°C ± 2°C |
ばく露装置の詳細 | phone in silence mode was placed in a wood bottom bamboo wire mesh cage (12 cm Œ 7 cm Œ 7 cm) in the center of the home cage with 3 rats; daily exposure composed of 50 unattended calls within 1 hour, each lasting for 45 seconds, followed by a 15-second pause interval before next call |
Sham exposure | A sham exposure was conducted. |
The level of thiobarbituric acid reactive substances was significantly increased in samples of exposed animals compared to sham exposure and cage control in all investigated brain regions. Moreover, exposure samples showed a significant decrease in the total antioxidative activity in the amygdala and cerebellum but it was not significantly changed in other brain regions. Glutathione S-transferase enzyme activity was significantly decreased in the hippocampus in the exposure group compared to sham exposure and cage control, but it was unaltered in other brain regions studied.
The authors conclude that an exposure of rats to a 900 MHz electromagnetic GSM field causes oxidative stress with regional differences in rat brain, which might be related to behavioral changes observed in Narayanan et al. 2013.
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