この研究は、携帯電話に慢性的にばく露されたラットの脳における酸化ストレスの強さを評価し、メラトニンに酸化ストレスおよび脳損傷を減少させる保護的効果の可能性があるか否かを調べた。携帯電話のテスト機(SAR=0.043-0.135W/kg)を用いて、Wisterラットに20、40、60日間のばく露実験を行った。各実験は、対照群(生食投与群)、メラトニン投与(Mel)群、マイクロ波ばく露(MW)群、MW+Mel群の4群で行った。その結果、MWばく露群では、MDAおよびカルボニル基群の著しい増加がみられ、40、60日ばく露後には、CATの減少とXOの活性増加が見られた;メラトニン処置は40日ばく露後のMDAレベルとXO活性の増加を阻害したが、CATの減少とカルボニル基群の増加を阻害することはできなかった、と報告している。
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To study the intensity of oxidative stress in the brain of animals chronically exposed to cell phones and potential protective effects of melatonin in reducing oxidative stress and brain injury.
Experiments were performed on 84 adult male rats exposed to microwave irradiation during 20, 40 and 60 days (4 hours/day during light period). Four groups (each 21 animals) were formed: 1) control-rats treated daily by saline, 2) rats treated daily with melatonin (2 mg/kg body weight), 3) microwave exposed rats and 4) microwave exposed rats treated with melatonin (2 mg/kg body weight).
周波数 | 900 MHz |
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タイプ |
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特性 |
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ばく露時間 | repeated daily exposure, 4 h/day, for 20, 40, and 60 days |
Modulation type | CW |
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ばく露の発生源/構造 |
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チャンバの詳細 | Seven animals were housed and exposed collectively in pure polycarbonate cages of 30 Œ 40 Œ 40 cm (W Œ L Œ H). |
ばく露装置の詳細 | Every four hours, two cages from the MW and MW+Mel groups were transferred to a wooden desk with rubber surface placed at 5 m from the housed rats, and a mobile test phone was put in a small perforated polycarbonate cell in the center of each cage preventing damage caused by rats. |
Sham exposure | A sham exposure was conducted. |
Additional information | Two dummy phones were put in the control and Mel group cages at the same time. |
A significant increase in the brain tissue malondialdehyde and carbonyl group concentration was registered during the whole exposure. Decreased enzyme activity of catalase and increased activity of xanthine oxidase remained after 40 and 60 days of exposure to mobile phones. Melatonin treatment significantly prevented the increase in the malondialdehyde content and xantine oxidase activity in the brain after 40 days of exposure while it was unable to prevent the decrease of catalase activity and increase of carbonyl group contents.
Mobile phones caused oxidative damage biochemically by increasing the levels of malondialdehyde, carbonyl groups, xanthine oxidase and decreasing catalase enzyme activity. Treatment with the melatonin significantly prevented oxidative damage in the brain.
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