研究のタイプ: 医学／生物学の研究 (experimental study)

[ラットにおける血清パラオキソナーゼ活性および脂質過酸化代謝に対する超低周波電磁界の影響] med./bio.

Effects of extremely low frequency electromagnetic fields on paraoxonase serum activity and lipid peroxidation metabolites in rat

著者: Seifirad S, Farzampour S, Nourbakhsh M, Amoli MM, Razzaghy-Azar M, Larijani B
掲載誌: J Diabetes Metab Disord 2014; 13 (1): 85 - 1-85 - 9
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この研究は、超低周波磁界（60Hz、0.5mT：ヘルムホルツコイル）ばく露により抗酸化機能障害と脂質過酸化上昇が生じて、アテローム生成的な影響が起きるのではないかという仮説の下、ラット実験でパラオキソナーゼ（PON）活性、抗酸化能、脂質過酸化代謝への影響を調べた。75匹のウィスターラットを5群に分けた：擬似ばく露群、一回ばく露群（血液検査：ばく露直後）一回ばく露群（ばく露の72時間後）、14日間ばく露群（直後）、14日間ばく露群（72時間後）。その結果、一回ばく露群では、脂質過酸化、PONを含む血清抗酸化活性の上昇が有意であった；慢性ばく露では、脂質過酸化が上昇、抗酸化系が影響を受けた､などの所見を報告している。

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研究目的（著者による）

The effects of a whole-body exposure of rats to a 60 Hz magnetic field on factors in blood which can provoke atherosclerosis should be investigated.

詳細情報

75 Wistar rats were randomly assigned to five groups: 1) sham exposure, 2) 4 h exposure, sacrificed immediately thereafter, 3) 4 h exposure, sacrificed 72 h thereafter, 4) 14 days of exposure, sacrificed immediately thereafter, and 5) 14 days of exposure, sacrificed 72 h thereafter. Blood samples were taken directly after sacrificing and analyzed.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: continuous for 4 hours
single exposure
ばく露2: 60 Hz
ばく露時間: continuous for 14 days
chronic exposure

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 4 hours
Additional information single exposure
ばく露装置
ばく露の発生源／構造
ばく露装置の詳細 A pair of Helmholtz coils set with winding surrounded in a plexi-glass frame. Through a variable transformer the coils were connected to a 220 V AC power supply. The centrally horizontal magnetic field was homogenous in a plexi-glass cage.To adjust the frequency and intensity, the coils were linked to a waveform generator.
Sham exposure A sham exposure was conducted.
Additional information Sham exposure group was placed in the same cage though the coils were turned off, being exposed to the local geomagnetic field (B-field, horizontal component = 0.018 mT, B-field vertical component = 0.022 mT, B-field total = 0.023 mT and B-field alternating current < 0.002 mT).
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 0.5 mT - 測定値 - -

ばく露2

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 14 days
Additional information chronic exposure
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 0.5 mT - 測定値 - -

Reference articles

  • Lee BC et al. (2004): [マウス脳の抗酸化防御系への低周波磁界の影響：化学発光研究]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

The results of all measured parameters of groups 2-5 were compared to the results of the sham exposure (group 1), respectively. A significantly higher paraoxonase enzyme activity, higher levels of conjugated diens, high density lipoprotein and free fatty acids as well as a significantly lower total antioxidative capacity could be observed in group 2 directly after the 4 h exposure. However, 72 h after the 4 h exposure (group 3), no significant alterations could be observed.
For group 4, significant changes of parameters comparable to the results of group 2 could be observed directly after 14 days of exposure, but additionally the level of malondialdehyde was significantly higher. 72 h after the 14-days exposure, the paraoxonase enzyme activity and the level of malondialdehyde were significantly higher and the total antioxidative capacity significantly lower (group 5).
The authors conclude that already a single exposure to a 60 Hz magnetic field with 0.5 mT has significant effects on factors in rat blood which can provoke atherosclerosis. However, these effects are reversible. The effects of a chronic exposure seem to be even more distinct and tend to be not reversible.

研究の種別:

研究助成

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