本研究は、先行研究で報告された、100μTの50Hz磁界への間欠ばく露によるヒト神経芽腫細胞株NB69の増殖促進が、より低い磁束密度10μTでも生じるかどうかを調べた細胞実験である。NB69細胞に対し、3時間オン／3時間オフで42時間、10または100μTの間欠ばく露の影響を、抗増殖剤のレチノイン酸の有無の場合について調べた。その結果、100μT の50Hz正弦波磁界への42時間のばく露がNB69細胞株の成長を促進するという、先行研究で報告されたデータが確認された；10μTでも同様の増殖応答が誘導された；この影響は増殖性細胞核抗原（PCNA）発現と有意に関連し、線形相関を示したが、培養液にレチノイン酸を添加した場合には阻害された、と報告している。
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To test whether a magnetic field of 10 µT and 100 µT influences the proliferation of a neuroblastoma cell line, alone or in combination with retinoic acid (a retinoid applied in oncostatic therapies).
A previous study (Trillo et al., 2012 ) has shown a stimulating effect of a magnetic field of 100 µT in a neuroblastoma cell line.
Two experiments were conducted: In the first experiment, cells were either exposed to a 10 µT magnetic field, exposed to a 100 µT magnetic field or sham exposed. In the second experiment the cell samples were submitted to one of the following treatment methods: 1.) sham exposure, 2.) sham exposure + retinoic acid (2 µM), 3.) exposure and 4.) exposure + retinoic acid (2 µM).
The cells were treated in the following groups: i) control group ii) retinoic acid treatment iii) exposure to EMF iv) retinoic acid treatment + exposure to EMF
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| 波形 |
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| ばく露時間 | intermittent for 3 h "on" - 3 h "off" for 42 h or 90 h |
| ばく露の発生源／構造 | |
|---|---|
| ばく露装置の詳細 | two sets of Helmholtz coils with a diameter of 20 cm and 1000 turns of enamelled copper wire each, positioned 10 cm apart; five or twenty Petri dishes placed between the coils for exposure; coil sets housed in magnetically shielded Co-Netic alloy boxes which were placed inside incubators with 5 % CO2 and 37°C |
| Sham exposure | A sham exposure was conducted. |
The experiments showed that exposure to magnetic fields of 10 µT or 100 µT significantly increased the proliferation and the DNA content when compared to the control samples, but did not influence the cell viability and the total protein content. No significant differences were observed between the two magnetic flux densities. However, the second experiment showed that a treatment with retinoic acid significantly reduced the proliferation in comparison to the control cell culture, independent if the cell cultures were exposed or sham exposed. Hence, no significant difference was observed between group 2 (sham exposure + retinoic acid) and group 4 (exposure + retinoic acid).
The authors conclude that a magnetic field exposure of 10 µT or 100 µT could enhance the cell proliferation in a neuroblastoma cell line and that retinoic acid may inhibit this effect. In summary, the present data could be of potential relevance to identify the mechanisms of action by which extremely low frequency magnetic fields affect human cells.
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