この実験研究は、ラットの脳に対する60Hz磁界(ばく露装置は直径30cmのヘルムホルツコイル。ケージを置いた空間の磁界強度2.4mT)の2時間急性ばく露の影響を調べた。Wistar雄ラット32匹を8匹ずつの4群に分けた(対照群、拘束(MR)した対照群、磁界ばく露群、磁界+MR群)。MRにはラット身体同等サイズのアクリル製シリンダーを用いた。ばく露後に屠殺して脳組織の分析を行った結果、磁界ばく露群ではカタラーゼとスーパーオキシドディスムターゼの活性低下が生じた;磁界+MR群では還元型グルタチオンと一酸化窒素レベルが低下した、などの知見が得られたが、急性磁界ばく露はストレスホルモンであるコルチコステロンの上昇を引き起こさなかったと報告している。
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To study the immediate effects of acute magnetic field exposure, immobilization, and the combination of both on the antioxidant system and lipid content in the whole brain of rat.
32 male rats were divided into four groups: 1) control group, 2) exposure group, 3) immobilization, 4) exposure + immobilization.
Immobilization was used as a positive control for physical and psychological stress.
ばく露 | パラメータ |
---|---|
ばく露1:
60 Hz
ばく露時間:
continuous for 2 h
|
|
Animals were divided into four groups: 1) control group (no exposure, no immobilization) 2) exposure group (no immobilization) 3) immobilization 4) exposure + immobilization
周波数 | 60 Hz |
---|---|
タイプ |
|
波形 |
|
ばく露時間 | continuous for 2 h |
ばく露の発生源/構造 | |
---|---|
ばく露装置の詳細 | rats were restrained by confinement into a 18 cm long acrylic cylinder with an inner diameter of 7 cm; unrestained rats were placed individually in 47 cm x 25 cm x 21 cm acrylic cages; pair of circular Helmholtz coils with an inner diameter of 30 cm, consisting of 350 turns of 18-gauge copper wire; coils separated by 15 cm from the upper and lower surfaces of the animal cage |
Sham exposure | A sham exposure was conducted. |
測定量 | 値 | 種別 | Method | Mass | 備考 |
---|---|---|---|---|---|
磁束密度 | 2.4 mT | - | 測定値 | - | - |
Acute exposure to extremely low frequency magnetic field alone as well as "exposure + immobilization" induced a reduction in catalase and superoxide dismutase enzyme activities compared with the control group, whereas the combination of "magnetic field exposure and immobilization" also decreased both reduced glutathione (compared with the control group) and nitric oxide levels compared with the other groups. Thiobarbituric acid reactive substances in the total lipid content of the brain were higher in the "exposure + immobilization group" than in the other experimental groups. Exposure to extremely low frequency magnetic fields did not induce any changes of the stress-hormone corticosterone or triacylglycerol levels.
Acute exposure to the extremely low frequency magnetic field appears to be a mild stressor.
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