研究のタイプ: 医学/生物学の研究 (experimental study)

[100および500μTのELF磁界はラット脳内のベータアミロイドタンパク、カルボニル化タンパク、マロンジアルデヒドに変化を与えるか?] med./bio.

Do 100- and 500-µT ELF magnetic fields alter beta-amyloid protein, protein carbonyl and malondialdehyde in rat brains?

掲載誌: Electromagn Biol Med 2013; 32 (3): 363-372

本研究は、超低周波(ELF)磁界ラット脳内のベータアミロイドタンパク(BAP)、カルボニル化タンパク(PC)、マロンジアルデヒドMDA)に及ぼす影響を調べた動物実験である。30匹の雄の成獣のSprague-Dawleyラットを2つのばく露群と偽ばく露群に分け、ばく露群はそれぞれ100、500μTの50Hz磁界に2時間/日、10か月間ばく露した。その結果、どちらのELF磁界もBAPレベルに有意に変化を生じなかった;どちらのELF磁界もPCおよびMDAレベルを増加させた、と報告している。

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研究目的(著者による)

To study the effects of 100 µT and 500 µT extremely low-frequency magnetic fields on levels of beta-amyloid protein, protein carbonyl and malondialdehyde in rat brain. The aim of this study was to illuminate the interaction between biomolecules and extremely low-frequency magnetic fields.

詳細情報

30 rats were divided into 3 groups: 2 exposure groups (100 µT and 500 µT) and 1 sham exposure group (each group n=10).

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: 2 h/day for 10 months
ばく露2: 50 Hz
ばく露時間: 2 h/day for 10 months

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 2 h/day for 10 months
ばく露装置
ばく露の発生源/構造
チャンバの詳細 exposures were carried out in methacrylate cages; rats moved freely in the cages during exposure
ばく露装置の詳細 pair of Helmholtz coils of 25 cm diameter in a Faraday cage (130 X 65 x 80 cm), with shielding grounded to protect against stray environmental electrical fields; coils were placed horizontally facing one another, and the distance between coils was 25 cm
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 100 µT spatial average 測定値 - -

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 2 h/day for 10 months
ばく露装置
ばく露の発生源/構造
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
磁束密度 500 µT spatial average 測定値 - -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

Protein carbonyl group and malondialdehyde levels were significantly increased by the exposure to 100 µT and 500 µT extremely low-frequency magnetic fields. However, beta-amyloid protein level was not significantly affected. In conclusion, the data showed that long-term exposure (2 h/day for 10 months) can affect some biological macromolecules such as proteins and lipids.

研究の種別:

研究助成

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