研究のタイプ: 医学／生物学の研究 (experimental study)

[超低周波磁界はラット脳に酸化ストレスを誘導する] med./bio.

Extremely low frequency magnetic fields induce oxidative stress in rat brain

著者: Manikonda PK, Rajendra P, Devendranath D, Gunasekaran B, Channakeshava, Aradhya SR, Sashidhar RB, Subramanyam C
掲載誌: Gen Physiol Biophys 2014; 33 (1): 81-90
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この研究は、超低周波磁界（ELF-MF）の長期ばく露を受けたラットの脳のさまざまな領域での酸化的ストレス（OS）を評価した。オスのウィスターラット（21日齢）に、ELF-MF（50 Hz、50および100 μT）ばく露を90日間連続して与え、ばく露後に、ラットの海馬、小脳、皮質領域の（i）活性酸素種（ROS）、（ii）OSを示す代謝物、（iii）抗酸化酵素を分析した。その結果、対照群に比べ、ELF-MFばく露群では、脳のすべての領域において、OSが引き起こされ、用量依存的にグルタチオン（GSH / GSSG）レベルが変化した；領域別にみると、ROSの蓄積、脂質過酸化最終産物の蓄積、およびスーパーオキシドジスムターゼの活性の大きさの程度は、小脳 < 海馬 < 皮質の順であった；GSH / GSSGレベル低下、グルタチオンペルオキシダーゼ活性上昇の程度は、海馬 < 小脳 < 皮質の順であった；50 μTばく露群に比べ、100 μTばく露群の方が、脳のすべての検査した領域で、OSをより有意に引き起こした、と報告している。

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研究目的（著者による）

To investigate the influence of a long-term exposure to a 50 Hz-magnetic field on oxidative stress in the rat brain.

詳細情報

Three groups of rats were examined (n= 6 per group): 1.) sham exposure, 2.) exposure to a magnetic field of 50 µT and 3.) exposure to a magnetic field of 100 µT.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 90 days
ばく露2: 50 Hz
ばく露時間: continuous for 90 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 90 days
ばく露装置
ばく露の発生源／構造
チャンバの詳細 animals were housed individually, cages were covered with a stainless steel mesh lid
ばく露装置の詳細 exposure system consisted of 18 wooden bobbins (0.5 x 0.5 x 0.5 m³); wherein individual bobbins were wound by two sets of horizontal coils (each set of 25 turns); individual turns in the horizontal coil were seperated by a distance of 5 mm; coils were made of 22 gauge enameled copper wire; distance of 0.3 m seperated the individual bobbins to avoid interference
Sham exposure A sham exposure was conducted.
Additional information a set-up similar to the exposed animals was build for the sham exposed ones: 18 wooden bobbins were established without energizing the coils (2.78 m between exposed and sham exposed set-up).
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 50 µT - 測定値 - -
Additional parameter details

geometric field inside the wooden boxes of control and exposed groups were: Z (vertical)= 15.894 ± 0.1418 µT and H (horizontal)= 39.43 ± 0.0131 µT

ばく露2

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 90 days
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 100 µT - 測定値 - -
Additional parameter details

geometric field inside the wooden boxes of control and exposed groups were: Z (vertical)= 15.894 ± 0.1418 µT and H (horizontal)= 39.43 ± 0.0131 µT

Reference articles

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

In the hippocampus and the cerebellum of exposed groups (50 µT and 100 µT), the lipid peroxidation and enzyme activity of the glutathione peroxidase were significantly increased compared to the sham exposed group, while in the cortex only the 100 µT-exposed group showed a significant increase. In the hippocampus, cerebellum and cortex, the enzyme activity of the superoxide dismutase was significantly increased in the 100 µT-exposed group in comparison to the sham exposed group, but not in the 50 µT-group. The GSH/GSSG ratio was significantly decreased in all examined brain areas of the 100 µT-exposed group compared to the sham exposure group, while in the 50 µT-exposure group the GSH/GSSG ratio was only significantly decreased in the cerebellum.
No significant differences between the groups were seen regarding the body weight.
The authors conclude that long-term exposure to a 50 Hz-magnetic field induces oxidative stress in the rat brain.

研究の種別:

研究助成

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