研究のタイプ: 医学／生物学の研究 (experimental study)

[50 Hz ELF電磁界短期ばく露はAT478げっ歯類扁平上皮がん細胞株におけるシスプラチン誘導性酸化応答を変化させる] med./bio.

Short-term exposure to 50 Hz ELF-EMF alters the cisplatin-induced oxidative response in AT478 murine squamous cell carcinoma cells

著者: Buldak RJ, Polaniak R, Buldak L, Zwirska-Korczala K, Skonieczna M, Monsiol A, Kukla M, Dulawa-Buldak A, Birkner E
掲載誌: Bioelectromagnetics 2012; 33 (8): 641-651
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この実験研究は、AT478げっ歯類扁平上皮がん細胞株における抗酸化酵素活性、脂質過酸化、DNA損傷レベル、活性酸素種（ROS）生成などに対するシスプラチンおよび超低周波電磁界（ELF-EMF）の影響を評価している。細胞株は24または75時間培養（それぞれシスプラチン添加の有無による2通り）とソレノイドコイルを用いた50 Hz/1 mT磁界の16分間ばく露の追加の有無別の合計8群で結果を比較したところ、シスプラチン添加群はROSレベルと抗酸化酵素活性が有意に上昇したが、それにELF-EMFばく露を追加した群ではROSレベルと抗酸化酵素活性は低下した；マロンジアルデヒド濃度は全ての群で有意に低下したが、特にシスプラチンとELF-EMFの両方にばく露した群で最大の低下であった；ELF-EMFばく露は、シスプラチンによるDNA損傷や酸化ストレスを小さくする効果があるなどの所見を報告している。

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研究目的（著者による）

To study the influence of cisplatin and an extremely low frequency magnetic field on antioxidant enzyme activity and the lipid peroxidation, as well as on DNA damage and reactive oxygen species production in AT478 carcinoma cells.

詳細情報

The cells were divided into the following eight groups: 1) control group, incubated for 24 h, 2) control group, incubated for 72 h, 3) magnetic field exposure, incubated for 24 h, 4) magnetic field exposure, incubated for 72 h, 5) cisplatin treatment, incubated for 24 h, 6) cisplatin treatment, incubated for 72 h, 7) magnetic field exposure + cisplatin treatment, incubated for 24 h and 8) magnetic field exposure + cisplatin treatment, incubated for 72 h.
Additionally, for the comet assay cells were treated in the following three groups: 1) treatment with H2O2, 2) magnetic field exposure + treatment with H2O2 and 3) magnetic field exposure + cisplatin treatment (incubation for 24 h) + treatment with H2O2 for 5 min. The comet assay was performed 0, 60 and 120 min after removal of the H2O2 solution.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 16 min

General information

Cells were divided into the following eight groups: 1) control, collected after 24 h 2) control, collected after 72 h 3) exposure to EMF, collected after 24 h 4) exposure to EMF, collected after 72 h 5) treated with cisplatin, collected after 24 h 6) treated with cisplatin, collected after 72 h 7) exposure to EMF + treated with cisplatin, collected after 24 h 8) exposure to EMF + treated with cisplatin, collected after 72 h for comet assay cells were additionally treated in the following three groups: 1) treatment with H2O2 2) exposure to EMF + treatment with H2O2 3) exposure to EMF for 16 min + treatment with cisplatin for 24 h + treatment with H2O2 for 5 min

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
ばく露時間 continuous for 16 min
ばく露装置
ばく露の発生源／構造
ばく露装置の詳細 40 cm long solenoid with a diameter of 20 cm consisting of 600 turns of 2 mm diamter copper wire wound in three forward-backward-forward continuous layers; solenoid placed in a water-jacketed, temperature and atmosphere controlled incubator; culture dishes inserted into a plexiglas cylinder and placed horizontally into the solenoid, so that the magnetic flux was parallel to the bottom
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 1 mT effective value 測定値 - -
Additional parameter details

local geomagnetic field: 37 µT AC background field: 2.09 µT

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Cells treated with cisplatin alone exhibited a significant increase in reactive oxygen species and antioxidant enzyme activities. The magnetic field exposure + cisplatin treatment resulted in decreased reactive oxygen species levels and antioxidant enzyme activity. A significant reduction in malondialdehyde concentrations was observed in all of the study groups, with the greatest decrease associated with cisplatin treatment + magnetic field exposure.
Cisplatin induced the most severe DNA damage; however, when cells were also exposed to the magnetic field, less DNA damage occurred. Exposure to the magnetic field alone increased the DNA damage compared to the control cells.
The extremely low frequency magnetic field exposure decreased the effects of oxidative stress and DNA damage induced by cisplatin; however, the extremely low frequency magnetic field exposure alone was also a mild oxidative stressor and DNA damage inducer. The authors hypothesize that extremely low frequency magnetic field exposure exerts differential effects depending on the exogenous conditions.

研究の種別:

研究助成

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