EMF-Portal | Reactions of keratinocytes to in vitro millimeter wave exposure

[Reaktionen von Keratinozyten auf Exposition mit Millimeterwellen in vitro]

Von: Szabo I, Rojavin MA, Rogers TJ, Ziskin MC

Veröffentlicht in: Bioelectromagnetics 2001; 22 (5): 358-364

Ziel der Studie (lt. Autor)

Hintergrund/weitere Details

Endpunkt

Exposition/Befeldung (teilweise nur auf Englisch)

Exposition	Parameter
Exposition 1: 61,2 GHz Modulationsart: CW Expositionsdauer: kontinuierlich für 15 oder 30 min, siehe add. information	Leistung: 20 mW (Ausgangsleistung) Leistungsflussdichte: 29 mW/cm² (± 2 mW/cm²) SAR: 770 W/kg (± 42 W/kg)

Exposition 1

Hauptcharakteristika

Frequenz	61,2 GHz
Typ	elektromagnetisches Feld
Expositionsdauer	kontinuierlich für 15 oder 30 min, siehe add. information
Zusatzinfo	± 2.1 GHz

Modulation

Modulationsart	CW

Expositionsaufbau

Expositionsquelle	Hornantenne rectangular 16 x 16 mm
Kammer	Exposures were performed in a shielded room (made of 12.5-mm thick low-carbon steel sheets) on a standard clean bench for tissue culture, and metal surfaces surrounding the horn antenna were covered with MW-absorbing material to minimize reflections. MW generator, power meter, and spectrum analyzer were located outside. [Rojavin et al., 1997, 1998]
Aufbau	Cell cultures in the 15-mm wells of a standard 24-well plate, placed on top of the antenna, were exposed, one well at a time. Four corner wells of each plate were loaded with keratinocyte suspension. Two wells were exposed to MW and two were left as plate controls.
Schein-Exposition	Eine Schein-Exposition wurde durchgeführt.
Zusatzinfo	Each corner well was individually exposed or sham exposed for 15 or 30 min, and the total exposure duration for each plate was 60 or 120 min.

Parameter

Messgröße	Wert	Typ	Methode	Masse	Bemerkungen
Leistung	20 mW	-	gemessen	-	Ausgangsleistung
Leistungsflussdichte	29 mW/cm²	-	berechnet	-	± 2 mW/cm²
SAR	770 W/kg	-	gemessen	-	± 42 W/kg

Mess- und Berechnungsdetails

The SAR was determined from the initial rate of temperature rise measured using a calibrated 0.1-mm thermocouple positioned in contact with the bottom of the well. The incident power density was calculated using the relationship described in [Gandhi and Riazi, 1986] from the SAR, density, penetration depth, and power reflection coefficient. The latter two quantities were measured using the method described in [Alekseev and Ziskin, 2000].

Referenzartikel

Alekseev SI et al. (2000): Reflection and absorption of millimeter waves by thin absorbing films
Rojavin MA et al. (1998): Antipruritic effect of millimeter waves in mice: evidence for opioid involvement
Rojavin MA et al. (1997): Electromagnetic millimeter waves increase the duration of anaesthesia caused by ketamine and chloral hydrate in mice
Gandhi OP et al. (1986): Absorption of millimeter waves by human beings and its biological implications

Methoden Endpunkt/Messparameter/Methodik

Untersuchtes System:

isolierte biol./chemische Substanz (in vitro)
intakte Zelle/Zellkultur (in vitro)

Untersuchungszeitpunkt:

nach der Befeldung

Hauptergebnis der Studie (lt. Autor)

Studienmerkmale:

medizinische/biologische Studie
experimentelle Studie
Voll-/Hauptstudie

Studie gefördert durch

National Institutes of Health (NIH), Maryland, USA

Richard J. Fox Foundation, USA

Themenverwandte Artikel

Kim DH et al. (2015): Effects of a continuous electromagnetic field on wound healing in human airway

Alekseev SI et al. (2010): Millimeter wave effects on electrical responses of the sural nerve in vivo

Millenbaugh NJ et al. (2008): Gene Expression Changes in the Skin of Rats Induced by Prolonged 35 GHz Millimeter-Wave Exposure

Aly AA et al. (2008): Effects of 900-MHz Radio Frequencies on the Chemotaxis of Human Neutrophils in Vitro

Beneduci A et al. (2007): Antiproliferative effect of millimeter radiation on human erythromyeloid leukemia cell line K562 in culture: ultrastructural- and metabolic-induced changes

Reactions of keratinocytes to in vitro millimeter wave exposure med./bio.