研究のタイプ: 医学／生物学の研究 (experimental study)

[In vitroでのミリ波ばく露へのケラチノサイトの反応] med./bio.

Reactions of keratinocytes to in vitro millimeter wave exposure

著者: Szabo I, Rojavin MA, Rogers TJ, Ziskin MC
掲載誌: Bioelectromagnetics 2001; 22 (5): 358-364
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この研究は、ヒトケラチノサイトに対するミリ波（MW）の影響を評価するために、HaCaTケラチノサイト細胞株を用いたインビトロ実験を行なった。MW誘導性のケラチノサイト機能変化を、増殖、接着、走化性、およびインターロイキン-1β（IL-1β）産生アッセイで測定した。ばく露条件は、周波数61.22 GHz、 SARは770 W/ kg、ばく露時間15 – 30分間とした。その結果、実験したばく露条件下では、自発的増殖、組織培養プレートへの接着、ランダム遊走、ならびにIL-8およびRANTES誘発による走化性は、MWばく露による影響を受けなかった；しかし、MWばく露は、IL-1βの細胞内レベルの中程度ではあるが有意な増加をもたらした；これらのデータは、ヒト皮膚（表皮の主成分はケラチノサイト）のMWばく露が、基底ケラチノサイトの活性化を引き起こし、IL-1β産生レベルを上昇させる可能性を示唆している、と報告している。

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研究目的（著者による）

To investigate the effects of millimeter wave exposure to human keratinocytes in vitro.

詳細情報

Proliferation, adhesion, migration, and RANTES- and interleukin-1-β-induced chemotaxis as well as interleukin-1-β production in human HaCaT keratinocyte cell line were studied.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 61.2 GHz
Modulation type: CW
ばく露時間: continuous for 15 or 30 min, see add. information

ばく露1

主たる特性
周波数 61.2 GHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 15 or 30 min, see add. information
Additional information ± 2.1 GHz
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
チャンバの詳細 Exposures were performed in a shielded room (made of 12.5-mm thick low-carbon steel sheets) on a standard clean bench for tissue culture, and metal surfaces surrounding the horn antenna were covered with MW-absorbing material to minimize reflections. MW generator, power meter, and spectrum analyzer were located outside. [Rojavin et al., 1997, 1998]
ばく露装置の詳細 Cell cultures in the 15-mm wells of a standard 24-well plate, placed on top of the antenna, were exposed, one well at a time. Four corner wells of each plate were loaded with keratinocyte suspension. Two wells were exposed to MW and two were left as plate controls.
Sham exposure A sham exposure was conducted.
Additional information Each corner well was individually exposed or sham exposed for 15 or 30 min, and the total exposure duration for each plate was 60 or 120 min.
パラメータ
測定量 値 種別 Method Mass 備考
電力 20 mW - 測定値 - output power
電力密度 29 mW/cm² - 計算値 - ± 2 mW/cm²
SAR 770 W/kg - 測定値 - ± 42 W/kg
測定および計算の詳細

The SAR was determined from the initial rate of temperature rise measured using a calibrated 0.1-mm thermocouple positioned in contact with the bottom of the well. The incident power density was calculated using the relationship described in [Gandhi and Riazi, 1986] from the SAR, density, penetration depth, and power reflection coefficient. The latter two quantities were measured using the method described in [Alekseev and Ziskin, 2000].

Reference articles

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

A statistically significant increase in the intracellular level of interleukin-1-β was observed. Cell proliferation, adhesion to tissue culture plates, migration, and cytokine induced chemotaxis were not affected by exposure.

研究の種別:

研究助成

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