研究のタイプ: 医学／生物学の研究 (experimental study)

[900MHz無線周波電磁界にばく露したマウスの適応応答：ブレオマイシン誘導DNA損傷および酸化的損傷とそれらの修復] med./bio.

Adaptive response in mice exposed to 900 MHz radiofrequency fields: Bleomycin-induced DNA and oxidative damage/repair

著者: Zong C, Ji Y, He Q, Zhu S, Qin F, Tong J, Cao Y
掲載誌: Int J Radiat Biol 2015; 91 (3): 270-276
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この研究は、マウスに900MHz無線周波（RF）電磁界ばく露（120 μW/cm2、1日4時間、7日間）を与え、その終了から4時間後にブレオマイシン（BLM）を投与して、マウスに適応応答が見られるか否かを検討した。RF単独群、BLM単独群、RF＋BLM群において、i) 白血球のDNA損傷、ii) 血漿、肝と肺の組織での酸化的損傷（MDA:マロンジアルデヒド）と抗酸化状態（SOD:スーパーオキシドジスムターゼ）を調べた。その結果、RF単独群では、BLM単独群とは対照的に、DNA損傷、酸化的損傷は増加しなかった；RF＋BLM群では、BLM誘導性のDNA損傷の低下が見られ、血漿と肝でのMDAレベル低下、肺でのSODレベル上昇が見られた､と報告している。

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研究目的（著者による）

It should be investigated if exposure of mice to a 900 MHz electromagnetic field induces an adaptive response with higher resistance against bleomycin.

詳細情報

Bleomycin causes damages in cells and tissue comparable to the damage caused by ionizing radiation.
The experiment was divided into two parts: In the first part, DNA damage was examined and in the second part, oxidative stress and the antioxidative status were examined. For the first part, 24 mice and for the second part, 48 mice were divided into 6 groups, respectively (each n=4 and n=8, respectively): 1) cage control, 2) sham exposure, 3) exposure to electromagnetic field only, 4) injection of bleomycin only, 5) sham exposure + bleomycin, 6) exposure + bleomycin. The experiment of the first part was conducted twice.
Bleomycin was administered 4 hours after the last exposure.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: CW
ばく露時間: continuous for 4 h/day for 7 days

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 4 h/day for 7 days
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
  • GTEMセル
チャンバの詳細 small plastic boxes
ばく露装置の詳細 single restrained mice were kept in small plastic boxes and exposed in an in-house built GTEM cell; the location and orientation of the animals in the GTEM cell during exposure was monitored
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
電力密度 120 µW/cm² - 測定値 - -
SAR 50 mW/kg - 計算値 - estimation

Reference articles

  • Cao Y et al. (2011): [適応応答の誘導：マウスの900 MHz RF電磁界への事前ばく露はその後の電離放射線ばく露による造血系損傷を減少させる]
  • Cao Y et al. (2010): [ガンマ線で誘導したマウスの造血系への損傷に対する900 MHzマイクロ波放射の影響]
  • McIntosh RL et al. (2010): [安静時の人体の温度評価のための包括的な組織特性データベース]
  • Gabriel C (1996): [RFおよびマイクロ波の周波数における身体組織の誘電特性のデータ蓄積]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
研究対象とした臓器系:
  • blood, liver, lung
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

No significant differences could be observed between cage control, sham exposure and exposure to the electromagnetic field alone (groups 1-3) in all experiments.
In mice of groups with bleomycin injection (4-6), a significant increase of DNA damage could be observed 20 minutes after the injection compared to groups 1-3. In consecutive measurements, a progressional decrease of DNA damage with increasing time was observed in groups 4-6, with significant lower DNA damage in the group with exposure to the electromagnetic field (group 6) compared to groups without exposure (groups 4 and 5).
Groups 4-6 showed significantly increased levels of malondialdehyde and significantly decreased levels of superoxide dismutase in all tissues compared to groups 1-3. Additionally, the level of malondialdehyde was significantly lower in plasma and liver and the level of superoxide dismutase significantly higher in the lung in group 6 compared to groups 4 and 5.
The authors conclude that exposure of mice to a 900 MHz electromagnetic field induces an adaptive response with higher resistance against genotoxic or oxidative effects of bleomycin and that this study provides some evidence on the mechanisms of action underlying such an adaption.

研究の種別:

研究助成

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