研究のタイプ: 医学／生物学の研究 (experimental study)

[非電離の無線周波電磁界にばく露したヒト血液リンパ球での適応応答：電離放射線誘発損傷に対する耐性] med./bio.

Adaptive response in human blood lymphocytes exposed to non-ionizing radiofrequency fields: resistance to ionizing radiation-induced damage

著者: Sannino A, Zeni O, Romeo S, Massa R, Gialanella G, Grossi G, Manti L, Vijayalaxmi, Scarfi MR
掲載誌: J Radiat Res 2014; 55 (2): 210-217
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この研究は、非電離の無線周波電磁界（RF）に事前ばく露したヒト末梢血リンパ球には、その後の電離放射線（XR）の高線量（チャレンジ量：CD）ばく露による遺伝損傷の誘発に抵抗性を示す適応応答（AR）が見られるか否かを調べた。4名の健康なドナーから採取した末梢血リンパ球を定法通り24時間培養した後、RFのアダプティブ量（AD；1950MHz、平均SAR 0.3 W/kg）に20時間ばく露した。採取後48時間で、X線のCDとして1.0 または1.5 Gyのばく露を行った。採取後72時間で、細胞を回収し小核（MN）の発生頻度を測定した。その結果、RF + XR（AD + CD）群では、XR単独（CD）群に比べ、リンパ球のMN数に有意な減少が見られた；RF誘発のARには、ドナーによる変動性が見られた､と報告している。

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研究目的（著者による）

To examine whether an exposure of human blood lymphocytes to radiofrequency fields exhibits an adaptive response by resisting genotoxic effects from subsequent exposure to X-radiation.

詳細情報

Adaptive response: Former studies have shown that different cell types exposed to an extremely small adaptation dose of a genotoxic agent are less susceptible to the induction of a genetic damage when given a higher challenge dose of the same or a similar genotoxic agent.
Blood lymphocytes of four male healthy donors were stimulated for 24 h with phytohaemagglutinin and then exposed for 20 hours to an adaptive dose of 1950 MHz radiofrequency field to a specific absorption rate of 0.3 W/kg or sham exposed. This was followed by a challenge dose of x-radiation (1.0 or 1.5 Gy). Cells were collected after 72 h total culture period and the frequency of micronuclei was recorded.
Further experiments were performed with X-radiation and mitomycin C as an adaptive dose. The results are not presented here.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,950 MHz
ばく露時間: continuous for 20 hours
  • SAR: 0.3 W/kg mean

ばく露1

主たる特性
周波数 1,950 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 20 hours
ばく露装置
ばく露の発生源／構造
チャンバの詳細 two exposure chambers of rectangular waveguides (WR 430) housed (together with sham waveguide) in commercial incubator; waveguides fed by amplified signal of a signal generator
ばく露装置の詳細 samples placed on four-layer plexiglass stand with 35 mm Petri dishes on each layer; the Petri dishes containing the diluted blood from each donor were kept in the outer positions and exposed to 0.3 W/kg, while dummy samples were placed in the inner positions of the incubator (37.0 ± 0.5°C, 95% air, 5% carbon dioxide)
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 0.3 W/kg mean 計算値 - -

Reference articles

  • Zeni O et al. (2012): [1950 MHz（UMTS）の無線周波放射は神経様PC12細胞の主要な影響評価項目に影響しない]
  • Zeni O et al. (2012): [無線周波電磁界にばく露されたヒト血液リンパ球における適応応答の誘導：ユニバーサルモバイル通信システム（UMTS）信号と比吸収率の影響]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Cell cultures exposed to a radiofrequency field as an adaptive dose before treated with a challenge dose of 1.5 Gy x-radiation (but not 1.0 Gy) showed a significant decrease in the number of micronuclei compared to those treated with x-radiation alone. However, a large variability was observed between the donors. The proliferation index was similar between cell cultures pre-exposed to an adaptive dose of radiofrequency fields and subsequently treated with X-radiation and those cell cultures treated with X-radiation alone.
The authors conclude that exposure of human lymphocytes to radiofrequency fields exhibits an adaptive response by resisting genotoxic effects from subsequent exposure to X-radiation.

研究の種別:

研究助成

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