この研究は、未熟及び成熟ラットの骨髄細胞に対する、45日間の高周波電磁界ばく露(1800MHz、SARは0.37Wkgまたは0.49W/kg)の細胞毒性作用、及び電磁界ばく露後15日間の回復期間での変化を調べた。その結果、未熟・成熟、SARに拘わらず、全てのばく露理群において、染色体異常(CA)、小核(MN)発生頻度、分裂指数(MI)及び多染性赤血球(PCEs)の割合に有意差が観察された;細胞毒性損傷は、未熟ラットでより顕著で、未熟ラットでは回復期間での改善は見られなかった、などの所見を報告している。
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To study the potential cytotoxic and genotoxic effects of long-term (45 days) exposure to 1800 MHz electromagnetic fields on bone marrow cells in immature and mature rats.
24 immature (two weeks old) and 24 mature (ten weeks old) rats were divided into six groups (each group n=8): 1) immature control group, 2) immature exposure group, 3) immature exposure group + recovery period of 15 days, 4) mature control group, 5) mature exposure group, 6) mature exposure group + recovery period of 15 days.
Bone marrow cells were collected from both femurs. Four rats were used for the micronucleus test and four rats for the chromosomal analysis and mitotic index.
ばく露 | パラメータ |
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ばく露1:
1,800 MHz
Modulation type:
pulsed
ばく露時間:
continuous for two hours/day for 45 days
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Rats were divided into six groups with eight animals each: i) immature control group ii) immature rats exposed to EMF iii) immature rats granted a recovery period of 15 days after exposure termination iv) mature control group v) mature rats exposed to EMF vi) mature rats granted a recovery period of 15 days after exposure termination
周波数 | 1,800 MHz |
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タイプ |
|
偏波 |
|
ばく露時間 | continuous for two hours/day for 45 days |
Modulation type | pulsed |
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Repetition frequency | 217 Hz |
ばく露の発生源/構造 |
|
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Distance between exposed object and exposure source | 4 cm |
チャンバの詳細 | pie cage plexiglas restrainer with twelve slices: one rat/slice; numerous holes for air condition at the top of the restrainer |
ばく露装置の詳細 | antenna (height: 15 cm) located in the middle of the pie cage; antenna fixed about 4 cm from the head (due to movements of rats, the distance ranged from 4 to 8 cm) |
Additional information | band interval of GSM modulator: 1750 - 1850 MHz |
The data showed significant differences in chromosome aberrations (increase), the micronucleus frequency (increase), mitotic index (decrease) and the ratio of polychromatic erythrocytes (decrease) in bone marrow cells of all exposure groups compared with the control group. Additionally, the cytotoxic and genotoxic damage was more remarkable in immature rats than in mature rats. The 15 days of recovery period was insufficient to compensate the genotoxic damage in immature rats after exposure.
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