研究のタイプ: 医学／生物学の研究 (experimental study)

[超低周波磁界はゼブラフィッシュ胚における発生毒性とアポトーシスを誘導する] med./bio.

Extremely Low-Frequency Magnetic Fields Induce Developmental Toxicity and Apoptosis in Zebrafish (Danio rerio) Embryos

著者: Li Y, Liu X, Liu K, Miao W, Zhou C, Li Y, Wu H
掲載誌: Biol Trace Elem Res 2014; 162 (1-3): 324-332
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この研究は、ゼブラフィッシュの胚発生に対する超低周波磁界（ELF-MF：50Hz；30、100、200、400、800μT；96時間連続ばく露）の影響を調べた。受精胚を、対照群、擬似ばく露群、5つの磁界強度のELF-MFばく露群の7群に分けた。その結果、ELF-MFばく露群では、孵化の遅れ、発生初期の心拍数減少が見られた；胚の死亡や異常は観察されなかった；アクリジンオレンジ染色により、主として腹部のひれと脊柱にアポトーシスが検出された､と報告している。

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研究目的（著者による）

To examine potential biological effects of extremely low frequency magnetic fields on the development of zebrafish embryos.

詳細情報

Fish embryos were divided into seven groups: 1.) no treatment ("cage control"), 2.) sham exposure, 3.) 30 µT magnetic field exposure, 4.) 100 µT magnetic field exposure, 5.) 200 µT magnetic field exposure, 6.) 400 µT magnetic field exposure and 7.) 800 µT µT magnetic field exposure. Directly after fertilization of the eggs, exposure was started.
Per group, three dishes with embryos were used. For every examination, 10 embryos were randomly chosen from each dish.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 96 hours
  • 磁束密度: 30 µT (less than 1% spatial variation (calculated))
  • 磁束密度: 100 µT (less than 1% spatial variation (calculated))
  • 磁束密度: 200 µT (less than 1% spatial variation (calculated))
  • 磁束密度: 400 µT (less than 1% spatial variation (calculated))
  • 磁束密度: 800 µT (less than 1% spatial variation (calculated))

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 96 hours
ばく露装置
ばく露の発生源／構造
チャンバの詳細 zebrafish embryos were cultured in dishes in the center of the coils
ばく露装置の詳細 square Helmholtz coils were used to generate the magnetic field, each consisting of 100 turns of 1.5-mm diameter copper wire; 50 Hz AC current was allowed to pass through a variable voltage transformer, producing a relatively homogeneous 50-Hz magnetic field; magnetic field strength was axial and was parallel to the coil axis
Sham exposure A sham exposure was conducted.
Additional information sham group was conducted with coils similar to those in the exposure system, but without electricity passing through the coils
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 30 µT - 測定値 - less than 1% spatial variation (calculated)
磁束密度 100 µT - 測定値 - less than 1% spatial variation (calculated)
磁束密度 200 µT - 測定値 - less than 1% spatial variation (calculated)
磁束密度 400 µT - 測定値 - less than 1% spatial variation (calculated)
磁束密度 800 µT - 測定値 - less than 1% spatial variation (calculated)
Additional parameter details

background magnetic field in the Laboratory was 0.13 ± 0.02 µT

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム（著者による）

No significant differences regarding mortality and malformations were found between the groups. However, in the exposed groups 5, 6, and 7 (magnetic flux density of 200 µT and higher) a significantly delayed hatching and a significantly decreased heart rate in the early developmental stages was observed compared to the control groups (group 1 and 2). In the exposed embryos of the groups 5, 6, and 7, considerable signs of apoptosis were found mainly in the ventral fin and spinal column, which were not present in the control embryos. This finding was confirmed by a significant up-regulation of caspase-3 in the exposed groups 5, 6, and 7 and of caspase-9 in group 7 in comparison to the control.
The authors conclude that extremely low frequency magnetic fields have a detrimental effect on the development of zebrafish embryos.

研究の種別:

研究助成

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