Studientyp:
Medizinische/biologische Studie
(experimentelle Studie)
Effects of 50 Hz sinusoidal electromagnetic fields of different intensities on proliferation, differentiation and mineralization potentials of rat osteoblasts
med./bio.
[Wirkungen von 50 Hz sinusförmigen elektromagnetischen Feldern in verschiedenen Stärken auf die Proliferation, Differenzierung und Mineralisation bei Ratten-Osteoblasten]
Von:
Zhou J, Ming LG, Ge BF, Wang JQ, Zhu RQ, Wei Z, Ma HP, Xian CJ, Chen KM
solenoid consisting of three cascade connected coils with an acrylic tube (inner diameter = 18 cm, height = 21 cm) as a common core; the number of windings of enamel copper wire in the middle coil was less than those in the bilateral coils; field homogeneous within 1% for an area of 30 mm to -30 mm from the horizontal and 20 mm to -20 mm from the vertical axis; solenoid positioned in an incubator at 37°C with 5 % CO2 and 100 % humidity; osteoblasts placed in 60 mm culture dishes inside the solenoid
t = 30 min/day for 3 days - for MTT assay t = 30 min/day for 3, 6, 9, 12 or 15 days - for alkaline phosphatase (ALP) activity t = 30 min/day for 8 days - for ALP histochemical staining t = 30 min/day for 10 days - for Alizarin red staining t = 30 min/day for 5, 8 or 10 days - for morphology of osteoblasts
Gansu Research Foundation of Science and Technology, China
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