Cells were exposed to TETRA RF fields in a perfusion chamber mounted on the stage of an inverted microscope within a stripline parallel-plate transmission line. The exposure system that is depicted with measures in a figure, was similar to that described in the reference article, but adapted to fit the stage of the inverted microscope. TETRA RF waveforms were provided by a function generator and an amplifier. The output waveform from the signal generator comprised an allocated frequency of 380.8875 MHz modulated according to the ETSI standard, to simulate that of in-service hand-sets. A directional coupler and power meter were used to monitor forward and reverse power. For exposures, the RF signal was fed into the transmission line and terminated (50 W termination). For sham exposures, the RF signal was fed directly into the termination, thus bypassing the transmission line. Cell cultures were randomly assigned to exposed or sham groups.
In order to investigate the effects of exposure intensity, cell cultures were exposed for consecutive 10-min periods to baseline and TETRA RF fields at 5, 10, 20 and 50 mW/kg.
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