この研究は、地上基盤無線(TETRA)電磁界が興奮性細胞の細胞内カルシウム信号伝達に影響を与えるか否かを細胞実験で調べた。培養ラット小脳顆粒細胞および心筋細胞における細胞内カルシウムイオン濃度[Ca(2+) ](i)を、TETRA電磁界(17.6 Hzでパルス変調された380.8875 MHz、パルスのデューティサイクル25 %)ばく露中に測定した。[Ca(2+) ](i)は、fura-PE3、fluo-3またはfluo-4蛍光としてデジタル画像分析により測定された。その結果、5、10、20、50または400 mW/ kgの比吸収率(SAR)でのばく露を受けた顆粒細胞では、静止時の[Ca(2 +) ](i)に有意な変化は見られなかった;カリウム誘発脱分極に反応した[Ca(2+) ](i)の上昇には、TETRAばく露群と擬似ばく露群で有意差が見られたが、差の大部分は、培養細胞間の初期の生物学的変動に起因するものであった;これらの細胞では、fura-PE3(UV励起)とfluo-3(可視光励起)の測定値に差がなかった;心筋細胞における自発的Ca(2+)トランジェントの速度または振幅のいずれにも、TETRA(50または400 mW/ kg)ばく露は影響しなかった;心筋細胞はサルブタモール(50 microM)およびアセチルコリン(10 microM)に対して正常な応答を示した、と報告している。
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To determine whether Terrestrial Trunked Radio (TETRA) fields can affect intracellular calcium signalling in excitable cells.
Cultured rat cerebellar granule cells and cardiac myocytes were loaded with the calcium-sensitive fluorescent probes fura- PE-3, fluo-3 (granule cells), and fluo-4 (cardiac myocytes).
The TETRA communication system incorporates a pulsed modulation at 17.6 Hz, very close to the 16 Hz modulation which has been reported to enhance calcium efflux (publication 309 and publication 8142).
ばく露 | パラメータ |
---|---|
ばく露1:
380.8875 MHz
Modulation type:
pulsed
ばく露時間:
continuous for 20 min
|
|
ばく露2:
380.8875 MHz
Modulation type:
pulsed
ばく露時間:
consecutive levels for a total of 40 min
|
|
ばく露3:
380.8875 MHz
Modulation type:
pulsed
ばく露時間:
continuous for 11 min
|
周波数 | 380.8875 MHz |
---|---|
タイプ |
|
ばく露時間 | continuous for 20 min |
Modulation type | pulsed |
---|---|
Duty cycle | 25 % |
Repetition frequency | 17.6 Hz |
ばく露の発生源/構造 |
|
---|---|
ばく露装置の詳細 | Cells were exposed to TETRA RF fields in a perfusion chamber mounted on the stage of an inverted microscope within a stripline parallel-plate transmission line. The exposure system that is depicted with measures in a figure, was similar to that described in the reference article, but adapted to fit the stage of the inverted microscope. TETRA RF waveforms were provided by a function generator and an amplifier. The output waveform from the signal generator comprised an allocated frequency of 380.8875 MHz modulated according to the ETSI standard, to simulate that of in-service hand-sets. A directional coupler and power meter were used to monitor forward and reverse power. For exposures, the RF signal was fed into the transmission line and terminated (50 W termination). For sham exposures, the RF signal was fed directly into the termination, thus bypassing the transmission line. Cell cultures were randomly assigned to exposed or sham groups. |
周波数 | 380.8875 MHz |
---|---|
タイプ |
|
ばく露時間 | consecutive levels for a total of 40 min |
Modulation type | pulsed |
---|---|
Duty cycle | 25 % |
Repetition frequency | 17.6 Hz |
周波数 | 380.8875 MHz |
---|---|
タイプ |
|
ばく露時間 | continuous for 11 min |
Modulation type | pulsed |
---|---|
Duty cycle | 25 % |
Repetition frequency | 17.6 Hz |
ばく露の発生源/構造 |
|
---|
The data showed no evidence of any consistent or biologically relevant effect of TETRA fields on intracellular calcium in granule cells and cardiac myocytes at any of the SAR tested.
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