研究のタイプ: 医学／生物学の研究 (experimental study)

[ヒトの上皮細胞の携帯電話によるhsp27/p38MAPKストレスの非熱活性化：がん関連とBBB関連効果の分子的メカニズム] med./bio.

Non-thermal activation of the hsp27/p38MAPK stress pathway by mobile phone radiation in human endothelial cells: Molecular mechanism for cancer- and blood-brain barrier-related effects

著者: Leszczynski D, Joenvaara S, Reivinen J, Kuokka R
掲載誌: Differentiation 2002; 70 (2-3): 120-129
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この研究は、ヒト内皮細胞株EA.hy926の培養物に、非熱的レベルの900 MHz GSM携帯電話マイクロ波放射を1時間与え、ストレス応答が活性化されるか否かを調べた。その結果、ばく露により、多数の、しかしその大部分は同定できないタンパク質のリン酸化状態が変化した；影響を受けたタンパク質の1つは、熱ショックタンパク質-27（hsp27）と同定された；ばく露で、hsp27のリン酸化の一時的増加が生じた；また、ばく露で、hsp27およびp38MAPKのタンパク質発現レベルに一時的変化が生じた；温度プローブでの計測が示すように、細胞培養の温度はばく露により変化しなかったので、これらのすべての変化は非熱的影響と考えられる、と報告している。

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研究目的（著者による）

To determine whether non-thermal exposure of cells to 900 MHz GSM mobile phone radiation activates signal transduction pathways and induces cellular stress response in a human model.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for 1 h
  • SAR: 2 W/kg mean (1.8-2.5 W/kg)

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 1 h
Modulation
Modulation type pulsed
Pulse width 0.577 ms
Repetition frequency 217 Hz
ばく露装置
ばく露の発生源／構造
  • モノポール
  • waveguide resonator
チャンバの詳細 The irradiation chamber was placed vertically inside a cell culture incubator.
ばく露装置の詳細 The modulated RF signal was amplified and fed to the exposure waveguide via a monopole type feed post. Two 55 mm glass Petri dishes were placed on a thin (9 mm) rectangular glass-fiber-molded temperature-controlled waterbed at the bottom of the chamber in such a way that the electric field vector was parallel to the plane of the culture medium.
Additional information The only difference between irradiated and sham samples was that the RF-EMF radiation was either turned on or turned off.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 2 W/kg mean 測定値および計算値 - 1.8-2.5 W/kg
測定および計算の詳細

The SAR distribution in the cell culture and the E-field above the cell culture were calculated using the FDTD method. The maximum SAR was obtained in the center of the Petri dish, decreasing by about 6 dB at the edges of the dish. Simulation results were verified with measurements. The electric field in the air above the cell cultures was measured with a calibrated miniature E-field probe. The measured and the simulated E-field values differed by less than 15%. The SAR distribution was measured with small, calibrated temperature probes directly from the culture medium of increased height at room temperature outside the incubator. The measured values can be considered as the upper and lower limits of SAR, due to measurement uncertainties, and thus, they validate the simulations.

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

The results suggest that mobile phone radiation activates cellular signal transduction and stress response pathways. 1-hour of non-thermal exposure of the cells changes phosphorylation status of numerous, yet largely unidentified, proteins. One of these proteins was heat shock protein 27 (hsp27). Mobile phone exposure caused a transient increase in phosphorylation of hsp27. This was prevented by a specific inhibitor of p38 mitogen-activated protein kinase (p38MAPK). Mobile phone caused also transient changes in the protein expression levels of p38MAPK and hsp27.

研究の種別:

研究助成

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