研究のタイプ: 医学／生物学の研究 (experimental study)

[ELF-EMFおよびRF-EMFのばく露は出芽酵母のグルコース輸送およびTCAサイクルの速度を上昇させる] med./bio.

Exposure of ELF-EMF and RF-EMF Increase the Rate of Glucose Transport and TCA Cycle in Budding Yeast

著者: Lin KW, Yang CJ, Lian HY, Cai P
掲載誌: Front Microbiol 2016; 7: 1378
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この研究は、出芽酵母をモデル生物として、50Hzの超低周波電磁界（ELF-EMF）および2.0GHzの無線周波電磁界（RF-EMF）ばく露に対する転写応答を、イルミナ（Illumina社）シーケンス技術を用いて調べた。その結果、ELF-EMFばく露では、28個の遺伝子の転写レベルが上昇、4個の遺伝子の転写レベルが低下した；RF-EMFばく露では、29個の遺伝子の転写レベルが上昇、24個の遺伝子の転写レベルが低下した;さらに実時間-定量ポリメラーゼ連鎖反応（RT-qPCR）法で検証すると、どちらのばく露の場合も、グルコース輸送およびTCAサイクルに関与する遺伝子の発現が増加していたが、解糖系に関与するものはなかった、と報告している。

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研究目的（著者による）

The effects of exposure of yeast cells to a 50 Hz or 2 GHz electro-magnetic field on the global gene expression should be investigated.

詳細情報

Cells were divided into 3 groups: 1) exposure to the 50 Hz field, 2) exposure to the 2 GHz field, 3) control group.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
ばく露時間: continuous for 96 hours
ばく露2: 2 GHz
Modulation type: CW
ばく露時間: continuous for 96 hours
  • SAR: 0.12 W/kg mean (cf. remarks) (for a single cell)
  • 電界強度: 20 V/m (at position of cells)

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • electric field
  • magnetic field
ばく露時間 continuous for 96 hours
ばく露装置
ばく露の発生源／構造
チャンバの詳細 plates placed between coils in incubator
ばく露装置の詳細 two combined solenoid systems were placed in incubator; each solenoid was 15 cm in length and 40 cm in diameter and was wrapped with 260 turns of single-strand copper wire; the distance between the closest ends of the two coils was 15 cm; temperature remained stable at 30°C
Additional information the incubator for the control group was placed in an area where the magnetic field was equal to the background level
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 6 mT - 測定値 - -
電界強度 205 V/m - 測定値 - -
Additional parameter details

in the sample area (round in shape with a diameter of 24 cm), the difference in the magnetic field strength was less than 5%

ばく露2

主たる特性
周波数 2 GHz
タイプ
  • electromagnetic field
波形
  • sinusoidal
ばく露時間 continuous for 96 hours
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
  • antenna
チャンバの詳細 plates
ばく露装置の詳細 the antenna of the exposure system was placed 24 cm above the sample area; the temperature was stable at 30°C
パラメータ
測定量 値 種別 Method Mass 備考
SAR 0.12 W/kg mean 計算値 cf. remarks for a single cell
電界強度 20 V/m - 測定値 - at position of cells
測定および計算の詳細

SAR was calculated with Finite Difference Time Domain

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

A total of 8 genes was found in group 1 (50 Hz exposure) which were significantly upregulated while 1 gene was significantly downregulated compared to the control group. In group 2 (2 GHz exposure), a total of 7 genes was significantly upregulated while 3 genes were significantly downregulated compared to the control group.
Further analysis revealed that both exposures significantly upregulated the expression of genes involved in glucose transportation and the citric acid cycle, but not the glycolysis pathway.
The authors conclude that exposure of yeast cells to a 50 Hz or 2 GHz electro-magnetic field might increase the gene expression rate of genes involved in cell metabolism.

研究の種別:

研究助成

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