研究のタイプ: 医学／生物学の研究 (experimental study)

[マウス海馬神経細胞由来HT22細胞におけるアミロイドβ誘発の細胞毒性に対する無線周波電磁界ばく露併用の影響マウス海馬神経細胞由来HT22細胞におけるアミロイドβ誘発の細胞毒性に対する無線周波電磁界ばく露併用の影響マウス海馬神経細胞由来HT22細胞におけるアミロイドβ誘発の細胞毒性に対する無線周波電磁界ばく露併用の影響] med./bio.

Effects of combined radiofrequency field exposure on amyloid-beta-induced cytotoxicity in HT22 mouse hippocampal neurones

著者: Lee JS, Kim JY, Kim HJ, Kim JC, Lee JS, Kim N, Park MJ
掲載誌: J Radiat Res 2016; 57 (6): 620-626
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この研究は、マウス海馬神経細胞由来HT22細胞において、アミロイドβ（Aβ）により誘発される細胞毒性に対し、無線周波電磁界ばく露（RF）を併用した場合の影響を調べた。まず、Aβ処置がHT22細胞増殖を濃度依存的に抑制することを確認した。一方、RFばく露は、細胞増殖に影響を与えず、またAβ誘発性の細胞増殖抑制に対してもほとんど影響しないことを確認した。さらにRF単独ばく露の場合、およびAβ処置とRFばく露を併用した場合について、細胞周期など詳細分析した結果、Aβにより誘発される細胞増殖抑制、ROS産生増加、細胞死の誘導に対し、RFばく露は有意な影響を与えなかったと報告している。

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研究目的（著者による）

The effects of exposure of mouse hippocampal neurons to combined 837 MHz and 1950 MHz electromagnetic fields on beta-amyloid protein-induced cytotoxicity should be investigated.

詳細情報

Few previous animal studies suggested beneficial effect of radiofrequency fields in Alzheimer's disease (Arendash et al. 2010, Banaceur et al. 2013). In contrast, EMF exposure was also found to increase beta-amyloid protein expression (Jiang et al. 2013). The present study should elucidate the possible mechanism of action in vitro.
Cells were divided into the following groups: 1) exposure to the electromagnetic fields only, 2) exposure to the electromagnetic fields and treatment with 5 µM beta-amyloid protein, 3) exposure to the electromagnetic fields and treatment with 50 µM beta-amyloid protein. For each group, a separate sham exposure group was used.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 837–1,950 MHz
ばく露時間: continuous for 2 hours

General information

cells were treated with respective concentrations of beta-amyloid protein (5 µM and 50 µM) for 30 minutes prior to exposure to the electromagnetic fields

ばく露1

主たる特性
周波数 837–1,950 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 2 hours
Modulation
Modulation type cf. additional information
Additional information

simultaneous CDMA signal at 837 MHz and W-CDMA signal at 1950 MHz
ばく露装置
ばく露の発生源／構造
  • conical antenna
Distance between exposed object and exposure source 13.6 cm
チャンバの詳細 100 mm petri dishes in exposure chamber with 37 ± 0.3°C and a 5% CO2
ばく露装置の詳細 radial transmission line (RTL) exposure system with conical antenna, which was located in the center of the exposure chamber; petri dishes were placed at 13.6 cm from the antenna
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 2 W/kg - 計算値 - CDMA signal
SAR 2 W/kg - 計算値 - W-CDMA signal
測定および計算の詳細

SAR distribution inside the exposed medium was characterized by numerical simulations using the Finite Difference Time Domain method

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Exposure to the electromagnetic fields had no significant effect on any parameter in any group compared to the respective sham exposure groups.
The authors conclude that exposure of mouse hippocampal neurons to combined 837 MHz and 1950 MHz electromagnetic fields has no effects on the beta-amyloid protein-induced cytotoxicity.

研究の種別:

研究助成

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