研究のタイプ: 医学/生物学の研究 (experimental study)

[MW放射はヒト神経芽細胞腫細胞株SH-SY5Yの遺伝子発現、アポトーシスレベルおよび細胞周期促進に影響するか?] med./bio.

Does MW Radiation Affect Gene Expression, Apoptotic Level, and Cell Cycle Progression of Human SH-SY5Y Neuroblastoma Cells?

掲載誌: Cell Biochem Biophys 2016; 74 (2): 99-107

この研究は、小児がん組織型の一種であるヒト神経芽細胞腫細胞株SH-SY5Yのアポトーシスレベル、細胞生存力および細胞周期促進に対するマイクロ波MW放射の影響を調べた。MWは、2.1GHzのW-CDMA変調波で、SARは0.491W/kg、ばく露時間は24時間とした。その結果、アポトーシスレベルにはばく露群と対照群で有意差がなかった;c-mycおよびcyclin D1のmRNAレベルはばく露群で有意に低下した;G1期の細胞割合は対照群に比べばく露群で有意に高かった、と報告している。

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研究目的(著者による)

The effects of exposure of neuroblastoma cells to a 2.1 GHz electromagnetic field (W-CDMA) on apoptosis and cell cycle progression should be investigated.

詳細情報

Neuroblastoma cells were used as a in vitro model for neural cells in embryos and infants, as it is assumed that these developmental stages are more sensitive to electromagnetic fields than adults.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 2.1 GHz
ばく露時間: continuous for 24 hours
  • 電界強度: 55 V/m
  • 電力: 0.316 W (antenna input power)
  • SAR: 0.235 W/kg minimum (1 g)
  • SAR: 0.491 W/kg maximum (1 g)
  • SAR: 0.35 W/kg mean (1 g)

ばく露1

主たる特性
周波数 2.1 GHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 24 hours
Modulation
Modulation type cf. additional information
Additional information

W-CDMA modulation

ばく露装置
ばく露の発生源/構造
チャンバの詳細 polystyrene plate with 24 wells in a CO2 incubator
ばく露装置の詳細 antenna which radiated vertically upwards on the well plate 1 mm above; the temperature inside the incubator was kept constant at 37°C
Sham exposure A sham exposure was conducted.
パラメータ
測定量 種別 Method Mass 備考
電界強度 55 V/m - 測定値 - -
電力 0.316 W - - - antenna input power
SAR 0.235 W/kg minimum 計算値 1 g -
SAR 0.491 W/kg maximum 計算値 1 g -
SAR 0.35 W/kg mean 計算値 1 g -

ばく露を受けた生物:

方法 影響評価項目/測定パラメータ/方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム(著者による)

The gene expressions of c-myc and cyclin D1 were significantly decreased and the percentage of cells in G1 phase was significantly higher in exposed cells compared to sham exposed cells, indicating cell cycle arrest in G1 phase.
There were no significant differences in apoptosis, cell viability or DNA fragmentation observed between exposed and sham exposed cells.
The authors conclude that exposure of neuroblastoma cells to a 2.1 GHz electromagnetic field (W-CDMA) has no effect on apoptosis but might change the cell cycle progression.

研究の種別:

研究助成

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