研究のタイプ: 医学／生物学の研究 (experimental study)

[低レベルのGSM 935 MHz無線周波電磁界へのばく露は増殖中または分化後のげっ歯類神経芽腫細胞にアポトーシスを誘導しない] med./bio.

Exposure to low level GSM 935 MHZ radiofrequency fields does not induce apoptosis in proliferating or differentiated murine neuroblastoma cells

著者: Moquet J, Ainsbury E, Bouffler S, Lloyd D
掲載誌: Radiat Prot Dosimetry 2008; 131 (3): 287-296
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この研究は、携帯電話に特徴的な無線周波（RF）電磁界が非熱的レベルで、増殖状態および分化状態のマウス神経芽細胞腫細胞株N2aにアポトーシスを誘導するか否かを調べた。細胞のばく露に用いた3タイプの935 MHz RF信号は、GSMの基本信号、GSMトーク信号、および連続波の非変調信号である。これらのうちのどれか１つに、SARレベル2 W/ kgで24 時間連続ばく露した。RFばく露による細胞温度の上昇の測定値は約0.06 ℃であった。ばく露後0〜48時間の多数の時点で、3つの独立したアッセイ（アネキシンV、カスパーゼの活性化、SEL法（in situ end labeling））を使用して、蛍光顕微鏡下で細胞のアポトーシスを評価した。その結果、ばく露細胞と擬似ばく露細胞の間にアポトーシスレベルの統計的有意差は観察されなかった、と報告している。

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研究目的（著者による）

This study was performed to examine whether radiofrequency fields characteristic of mobile phones at non-thermal levels can induce apoptosis in murine neuroblastoma cells in both proliferating and differentiated states.

詳細情報

The positive controls were X-ray (4 Gy) treated cells.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 935 MHz
Modulation type: pulsed
ばく露時間: continuous for 24 h
  • SAR: 2 W/kg mean
ばく露2: 935 MHz
Modulation type: pulsed
ばく露時間: continuous for 24 h
  • SAR: 2 W/kg mean
ばく露3: 935 MHz
Modulation type: CW
ばく露時間: continuous for 24 h
  • SAR: 2 W/kg mean

General information

The complete detail of the exposure apparatus, the signal profile, the exposure geometry and dosimetry have been described in [Schuderer et al., 2004].

ばく露1

主たる特性
周波数 935 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 24 h
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional information

GSM basic
ばく露装置
ばく露の発生源／構造
チャンバの詳細 Two identical waveguide cavities were placed in a tissue culture incubator maintained at 37°C with a humidified atmosphere of 5% CO2 in air. They were randomly assigned for RF or sham exposure. Good exposure and environmental control was achieved using field sensors, air temperature sensors, and an optimised airflow system.
ばく露装置の詳細 Cells were seeded into 35-mm Petri dishes in 3 ml of growth medium 24 h before any treatment and were placed inside the waveguides one hour before exposure. A specially designed holder allowed eight 35-mm Petri dishes to be placed accurately inside each chamber.
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 2 W/kg mean - - -
Additional parameter details

Cells irradiated at room temperature with 4.0 Gy of 250 kVp X-rays at a dose rate of 1.0 Gy/min were used as a positive control together with a zero dose control.

ばく露2

主たる特性
周波数 935 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 24 h
Modulation
Modulation type pulsed
Repetition frequency 217 Hz
Additional information

GSM talk (random changes between GSM basic and GSM DTX)
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 2 W/kg mean - - -

ばく露3

主たる特性
周波数 935 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 24 h
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 2 W/kg mean - - -

Reference articles

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
研究対象とした臓器系:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

No statistically significant differences in apoptosis levels were found between the exposed and sham-exposed murine neuroblastoma cells neither in proliferating nor in differentiated state using the three assays at any time point post-radiofrequency irradiation.

研究の種別:

研究助成

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