この研究は、多形性膠芽腫（GBM）の細胞株（U87）のイン・ビトロでの細胞周期、アポトーシスおよび生存率に対する超低周波パルス電磁界（ELF-PEMF）の影響を周波数、振幅およびばく露時間を変えて調べた。実験は、3通りのばく露時間（2、4、24時間）×5通りのばく露条件（I（無ばく露対照）、II（50Hz、100G）、III（100Hz、100G）、IV（10Hz、50G）、V（50Hz、50G））の15通りとした。ばく露終了後に、形態学的特性、細胞生存率、細胞周期およびアポトーシス関連タンパク質の遺伝子発現（Cyclin-D1、P53およびCaspase-3）を調べた。その結果、24時間ばく露では、II群で生存率およびCyclin-D1発現の増加が見られた一方、IIIおよびIV群ではそれらは減少した；またIII群ではp53およびCaspase-3発現が増加した；V群では何も変化はなかった､などの所見を報告している。
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The effects of exposure of glioblastoma cells to a pulsed extremely low frequency magnetic field on cell cycle, apoptosis and cell viability should be investigated in view of a possible use in cancer therapy.
Cells were divided into the following groups: exposure to a magnetic field with 1) 50 Hz/10 mT, 2) 100 Hz/10 mT, 3) 10 Hz/5 mT, 4) 50 Hz/5 mT and 5) control group. Each group was investigated after 2, 4 and 24 hours of exposure.
| 周波数 | 50 Hz |
|---|---|
| タイプ |
|
| ばく露時間 | for 2, 4 and 24 hours |
| Additional information | pulsed and/or continuous square waves (?) |
| ばく露の発生源／構造 | |
|---|---|
| ばく露装置の詳細 | polyvinyl chloride (PVC) cell culture flasks or plates with 96 wells were placed between two identical coils in a vertical field: each coil was boxed in a plexiglass chamber with dimensions of 160 x 160 x 50 mm3 by maintaining a coaxial distance of 40 mm; cooling water system around coils avoided temperature rise; cells were always placed on the same shelves of the cell culture incubator |
| 測定量 | 値 | 種別 | Method | Mass | 備考 |
|---|---|---|---|---|---|
| 磁束密度 | 10 mT | - | 測定値 | - | ± 1.5 mT |
The most distinct effects were found after 24 hours of exposure. Here, the cell viability and Cyclin-D1 protein expression were significantly increased in group 1 compared to the control group, while they were significantly decreased in groups 2 and 3. Protein expression of P53 and Caspase-3 was significantly increased in group 2 after 24 hours compared to the control group.
Cell morphology showed significant alterations in groups 1-3 after 24 hours compared to the control group with decreased mass, increased cell surface and changed shape.
The authors conclude that exposure of glioblastoma cells to a pulsed extremely low frequency magnetic field may alter cell cycle, apoptosis and cell viability depending on exposure duration, field frequency and intensity.
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