この研究は、ヒト神経芽細胞腫細胞株SH-SY5Yに対する超低周波磁界(50Hz:50 μT、0.8 mT、1.4 mT)の影響を調べた。ばく露は、培養器内に設置したヘルムホルツコイルの中央の一様磁界中に細胞を置き、4時間ばく露した。対照群はコイルを設置していない別の培養器で培養した。ばく露後、フーリエ変換赤外分光(FTIR)を用いてSH-SY5Yの全反射吸収(ATR)スペクトラムを測定した。その結果、50 μTばく露群で、アミドAバンドの強度低下、メチレン基に対応する2921 cm-1 および2853 cm-1 での振動バンドの強度の小さな低下が見られた;0.8 mTおよび1.4 mTばく露群では、CH2 振動バンドの強度の明確な上昇が見られた;また0.8 mTおよび1.4 mTばく露群では、アミドI領域でタンパク質構造変化に関係する変化が見られた、と報告している。
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To investigate whether exposure to extremely low frequency magnetic fields influences the mitochondrial transmembrane potential in neuroblastoma cells and to study the relationship between conformational changes of biological molecules and cell damage.
周波数 | 50 Hz |
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タイプ |
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ばく露時間 | continuous for 4 hours |
ばく露の発生源/構造 | |
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チャンバの詳細 | cells were exposed in 25 cm² culture flasks or in 96-well plates |
ばく露装置の詳細 | samples were placed between the coils at the centre of a uniform field area; exposure system consisted of a couple of Helmholtz coils, with pole pieces of round polar faces; coil radius was 150 mm; coils were put in incubator ( 5% CO2, 95% humidity, 37.1°C) |
Sham exposure | A sham exposure was conducted. |
In cell cultures exposed to magnetic flux densities of 0.63 mT or higher, the cell viability was signicantly decreased compared to the control group. Additionally, in cells exposed to a magnetic flux density of 1.41 mT, the transmembrane potential was significantly decreased in comparison to the control.
The exposure of 0.81 mT and higher led to significantly changed absorption spectra of the functional groups. These changes suggest conformational changes in proteins, DNA and lipids.
The authors conclude that exposure to extremely low frequency magnetic fields led to conformational changes of different biological molecules in neuroblastoma cells and that these alterations can be associated with a reduction of the mitochondrial transmembrane potential and the cell viability.
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