研究のタイプ: 医学／生物学の研究 (experimental study)

[神経2a細胞に900MHz無線周波電磁放射が誘発するDNA塩基の酸化的損傷の感受性は8-oxoG DNAグリコシラーゼ1の阻害により高められる] med./bio.

8-oxoG DNA Glycosylase-1 Inhibition Sensitizes Neuro-2a Cells to Oxidative DNA Base Damage Induced by 900 MHz Radiofrequency Electromagnetic Radiation

著者: Wang X, Liu C, Ma Q, Feng W, Yang L, Lu Y, Zhou Z, Yu Z, Li W, Zhang L
掲載誌: Cell Physiol Biochem 2015; 37 (3): 1075-1088
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この研究は、8-oxoG DNAグリコシラーゼ1（OGG1）の阻害の有無の条件下で、無線周波電磁界（RF-EMFs）の24時間ばく露中の神経2a細胞に生じると推定されるイン・ビトロでの遺伝毒性を調べた。RF-EMFsは、900MHz GSMの通話モードの信号、SARは0、0.5、1、2W/kgとした。DAN鎖切断、DNA塩基損傷、活性酸素種（ROS）レベル、細胞の生存率を評価した。その結果、OGG1は、DNA塩基の修復過程に関与しており、RF-EMFsによる酸化的損傷からDNA塩基を保護する役割を持つという仮説が支持されることが示された、と報告している。

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研究目的（著者による）

The genotoxic effects of exposure of neuroblastoma cells to a 900 MHz electromagnetic field (GSM) and the role of the oxoguanine glycosylase should be investigated.

詳細情報

Oxoguanine glycosylase (also called 8-oxoG DNA glycosylase-1) is the major protective enzyme against the mutagenic effects of 8-oxoguanine (similar to 8-OHdG). 8-Oxoguanine DNA lesions result from reactive oxygen species. If the oxoguanine glycosylase plays a role in radiofrequency electromagnetic field-induced oxidative DNA damage should be investigated in the study. Therefore, the enzyme was partly disabled via transfection with siRNA ("gene silencing").
Cells were exposed to 1) SAR of 0.5 W/kg, 2) SAR of 1 W/kg, 3) SAR of 2 W/kg. For each of these groups, a separate sham exposure was conducted. Additionally, a negative control and a positive control were conducted. Groups 2 and 3 and their respective sham exposure groups were also investigated with silencing of the oxoguanine glycosylase.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for 24 hours
  • SAR: 0.5 W/kg mean (SAR variability < 6%)
ばく露2: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for 24 hours
  • SAR: 1 W/kg mean (SAR variability < 6%)
ばく露3: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for 24 hours
  • SAR: 2 W/kg mean (SAR variability < 6%)

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 24 hours
Modulation
Modulation type pulsed
Additional information

GSM Talk signal
ばく露装置
ばく露の発生源／構造
チャンバの詳細 petri dishes in two rectangular chambers placed inside the same incubator with a common location for the air inlet to maintain the same environmental conditions (37°C, 5% CO2)
ばく露装置の詳細 one of the chambers was excited by an EMF signal mimicking the basic pulse structure of the GSM Talk signal at 900 MHz and the other was used for sham exposure (random choice); 8 petri dishes with cell monolayers were arranged in two towers of four dishes each; the temperature increased by 0.02°C/(W/kg) of the average SAR value during exposure; the temperature difference between exposed and sham-exposed chambers did not exceed 0.1°C
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 0.5 W/kg mean - - SAR variability < 6%

ばく露2

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 24 hours
Modulation
Modulation type pulsed
Additional information

GSM Talk signal
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 1 W/kg mean - - SAR variability < 6%

ばく露3

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
ばく露時間 continuous for 24 hours
Modulation
Modulation type pulsed
Additional information

GSM Talk signal
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 2 W/kg mean - - SAR variability < 6%

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Without oxoguanine glycosylase silencing, only group 3 (2 W/kg) showed a significantly increased amount of DNA base damage and reactive oxygen species in exposed cells compared to sham exposed cells.
Oxoguanine glycosylase silencing caused significantly increased amounts of DNA base damage in exposed cells of groups 2 (1 W/kg) and 3 compared to exposed cells of the respective group without oxoguanine glycosylase silencing.
No effects on DNA strand breaks and on cell viability were found in any group with or without oxoguanine glycosylase silencing.
The authors conclude that exposure of neuroblastoma cells to a 900 MHz electromagnetic field (GSM) might induce oxidative DNA base damage. The oxoguanine glycosylase could play a critical role in the protection of cells against this damage.

研究の種別:

研究助成

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