研究のタイプ: 医学／生物学の研究 (experimental study)

[携帯電話周波数帯(835.62、847.74 MHz)の電磁放射へのばく露後のDNA損傷測定] med./bio.

Measurement of DNA damage after exposure to electromagnetic radiation in the cellular phone communication frequency band (835.62 and 847.74 MHz)

著者: Malyapa RS, Ahern EW, Straube WL, Moros EG, Pickard WF, Roti Roti JL
掲載誌: Radiat Res 1997; 148 (6): 618-627
RISフォーマットで参考文献をダウンロード

この研究は、マウスC3H 10T1/2線維芽細胞およびヒトグリア芽細胞腫U87MG細胞を用いて、インビトロ培養物への携帯電話通信周波数放射により、DNA損傷が生じるか否かを調べた。ばく露には、搬送周波数835.62MHzの周波数変調連続波（FMCW）、および中心周波数847.74MHzの符号分割多元接続（CDMA）の2種類の周波数変調波を用いた。指数関数的増殖期（U87MGおよびC3H 10T1/2細胞）およびプラトー期（C3H 10T1/2細胞）の培養物に、FMCWまたはCDMA放射のいずれかを、24時間までの様々なばく露時間のばく露を与えた。SAR 0.6W / kgにおいて比較的一様なばく露が行えるよう設計された放射伝送路（RTL）内でばく露を行った。RTL中の温度は37±0.3℃に維持した。RTL内での擬似ばく露を受けた試料（陰性対照）および137Csガンマ線照射試料（陽性対照）を各実験に含めた。アルカリ性コメットアッセイでDNA損傷を測定した。その結果、FMCWまたはCDMAばく露群と陰性対照群との間に有意差は観察されなかった、と報告している。

The detailed summary of this article is not available in your language or incomplete. Would you like to see a complete translation of the summary? Then please contact us →

研究目的（著者による）

Mouse fibroblast cells and human glioblastoma cells were exposed to cellular phone communication frequency radiations to investigate whether such exposure produces DNA damage in in vitro cultures.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 835.62 MHz
Modulation type: FM, CW
ばく露時間: continuous for 2, 4 or 24 h
  • 電力: 60 W (50 to 60 W net forward power)
  • SAR: 0.6 W/kg mean (± 0.3 W/kg)
ばく露2: 847.74 MHz
Modulation type: pulsed
ばく露時間: continuous for 2, 4 or 24 h
  • 電力: 60 W (50 to 60 W net forward power)
  • SAR: 0.6 W/kg mean (± 0.3 W/kg)

ばく露1

主たる特性
周波数 835.62 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 2, 4 or 24 h
Modulation
Modulation type FM, CW
Additional information

FMCW
ばく露装置
ばく露の発生源／構造
チャンバの詳細 The radial transmission line (RTL) facility has been described in the reference article. In addition, the RTLs and the housings were shielded to avoid interference with mobile communication services.
ばく露装置の詳細 One T-75 culture flask was used for each of the times in each experiment. All experiments were carried out with matched sham-treated controls, except for U87MG cells where only one group was sham-exposed for 4 hours.
Additional information Sham-exposure (negative control) and 137Cs γ-irradiated samples (positive control, see reference article) were included with every experiment. A separate series of experiments was carried out with additional BrdU labelling of cells. One of these cultures (positive control) was not irradiated but only placed 50 cm under a 30-W fluorescent light source for 1 hour.
パラメータ
測定量 値 種別 Method Mass 備考
電力 60 W - - - 50 to 60 W net forward power
SAR 0.6 W/kg mean 測定値および計算値 - ± 0.3 W/kg
測定および計算の詳細

An extensive analytical, numerical and experimental dosimetric evaluation was performed using a two-dimensional frequency domain seven-layer model and a fast temperature differential measurement technique. The experimental result was in reasonable agreement with the calculation. A fully loaded RTL with 16 T-75 flasks, each containing 40 ml of culture medium with their long axis parallel to and equidistant from the antenna produced an average SAR over the bottom of the flask of 11.2 ± 5.6 mW/kg per 1 W of net forward power or 7.5 ± 3.8 mW/kg per 1 mW/cm² power incident at the proximal end of the flask. The values obtained were scaled to the net forward power applied to each radiating RTL.

ばく露2

主たる特性
周波数 847.74 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 continuous for 2, 4 or 24 h
Modulation
Modulation type pulsed
Duty cycle 40 %
Additional information

CDMA
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
パラメータ
測定量 値 種別 Method Mass 備考
電力 60 W - - - 50 to 60 W net forward power
SAR 0.6 W/kg mean 測定値および計算値 - ± 0.3 W/kg

Reference articles

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

No significant differences were observed between the test group exposed to FMCW or CDMA radiation and the sham-exposed negative controls. The results indicate that exposure of cultured mammalian cells to cellular phone communication frequencies does not cause DNA damage.

研究の種別:

研究助成

関連論文