研究のタイプ: 医学／生物学の研究 (experimental study)

[間葉系幹細胞の神経分化に対する流体剪断応力と電磁界との組み合わせの影響] med./bio.

Combined effects of flow-induced shear stress and electromagnetic field on neural differentiation of mesenchymal stem cells

著者: Mascotte-Cruz JU, Rios A, Escalante B
掲載誌: Electromagn Biol Med 2016; 35 (2): 161-166
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この研究は、骨髄由来の間葉系幹細胞（MSCs）に、流体が負荷となって生じる剪断応力（FSS）と電磁界とを同時に1時間与え、その後の神経への分化への影響を調べた。その結果、両者の処置後9時間の培養で、神経前駆体細胞のマーカであるネスチンの存在が確認され、その後6日間、発現が継続した､と報告している。

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研究目的（著者による）

The effects of co-exposure of rat bone marrow-derived mesenchymal stem cells to a 60 Hz magnetic field and flow-induced shear stress on neural differentiation should be investigated.

詳細情報

Individual exposure to flow-induced shear stress (mechanical stress) or magnetic fields have shown to be beneficial in neural differentiation of cells (see Cho et al. 2012 and Kim et al. 2013). However, a combination of both measures has not been investigated to date and the authors supposed a synergistic effect of co-exposure.
Cells were divided into the following groups: 1) co-exposure to the magnetic field and flow-induced shear stress, 2) exposure to the magnetic field, 3) exposure to flow-induced shear stress, 4) control group.
After exposure, cells were re-cultured and investigated after 1 hour, 9 hours, 1 day, 3 days and 6 days of incubation.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 60 Hz
ばく露時間: continuous for 1 hour

General information

fluid shear stress in groups 1 and 3 was 15 dynes/cm2

ばく露1

主たる特性
周波数 60 Hz
タイプ
  • magnetic field
波形
  • sinusoidal
ばく露時間 continuous for 1 hour
ばく露装置
ばく露の発生源／構造
ばく露装置の詳細 solenoid had a length of 10 cm, 400 loops and a radius of 2.5 cm; it was placed into a water jacket to maintain constant temperature of 37°C; system was combined with shear force system and cells flowed constantly through a microfluidic chamber placed on top of the solenoid
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 17.01 mT - 測定値 - -
Additional parameter details

no electric emission from solenoid was detected

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Cells either exposed to the magnetic field (group 2) or flow-induced shear stress (group 3) as well as cells from the control group (group 4) showed a flattened, spindleshaped and fibroblast-like morphology. By contrast, in the co-exposure group (group 1), some cells exhibited narrow, elongated, neurite-like branches, i.e. a neural phenotype.
The nestin protein expression was significantly increased in group 1 compared to the control group 1, 3 and 6 days after exposure. All other groups showed only a scarce nestin protein expression. CD31 was only expressed in 1% of all cells in group 1, indicating that there were almost no endothelial cells present.
The authors conclude that co-exposure of rat bone marrow-derived mesenchymal stem cells to a 60 Hz magnetic field and flow-induced shear stress might have a synergistic effect which promotes neural differentiation.

研究の種別:

研究助成

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