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The effects of exposure of human stem cells to a 50 Hz magnetic field on neuronal differentiation and the involvement of the NMDA receptor should be investigated.
Cells were divided into an exposure group and a sham exposure group. To investigate the role of the NMDA receptor, the cells were partially treated with memantine, an NMDA receptor antagonist.
ばく露 | パラメータ |
---|---|
ばく露1:
50 Hz
ばく露時間:
continuous for 5 days
|
|
周波数 | 50 Hz |
---|---|
タイプ |
|
波形 |
|
ばく露時間 | continuous for 5 days |
ばく露の発生源/構造 | |
---|---|
ばく露装置の詳細 | Helmholtz coil pair placed inside a cell culture incubator; power supply signal was attenuated by a variac and fed to the coils to produce magnetic field |
Sham exposure | A sham exposure was conducted. |
測定量 | 値 | 種別 | Method | Mass | 備考 |
---|---|---|---|---|---|
磁束密度 | 1 mT | effective value | 測定値 | - | - |
Both immunohistochemical staining and Western blot showed a significant increase in neuronal differentiation of cells in the exposure group compared to the sham exposure group. The differentiated neurons in the exposure group also showed significantly longer axons and a significantly increased level of c-Fos compared to the sham exposure group. Addition of the NMDA receptor inhibitor memantine reversed these effects.
The authors conclude that exposure of human stem cells to a 50 Hz magnetic field could promote neuronal differentiation with the involvement of the NMDA receptor.
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