研究のタイプ: 医学／生物学の研究 (experimental study)

[毛包真皮乳頭細胞でのMAP2およびネスチン遺伝子の発現に対する超低周波電磁界の影響] med./bio.

Effect of extremely low frequency electromagnetic field on MAP2 and Nestin gene expression of hair follicle dermal papilla cells

著者: Moraveji M, Haghighipour N, Keshvari H, Nourizadeh Abbariki T, Shokrgozar MA, Amanzadeh A
掲載誌: Int J Artif Organs 2016; 39 (6): 294-299
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この研究は、毛包の真皮乳頭細胞（DPCs）でのMAP2およびネスチン遺伝子の発現に対する超低周波電磁界（ELF-EMF：50 Hz, 1 mT）の影響を調べた。4つの実験条件（ELF-EMF単独ばく露、化学的因子（薬剤名は不記載）単独ばく露、化学的因子＋ELF-EMFばく露、対照（無処置））で、それぞれ5日間ばく露を行った。また、EMF単独ばく露についてはばく露期間を14日間に延長もした。その結果、EMFの5日間ばく露に比べ、14日間のばく露において、MAP2発現が増加し、細胞増殖が低下した、とのみ報告している。

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研究目的（著者による）

The effects of exposure of dermal papilla mesenchymal cells to a pulsed 50 Hz magnetic field on the gene expression of neural differentiation markers should be investigated.

詳細情報

Cells were divided into the following groups: 1) exposure to magnetic field for 5, 14 and 20 days, 2) treatment with neural differentiation medium for 5 days, 3) co-exposure to the magnetic field and neural differentiation medium for 5 days and 4) control group.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 50 Hz
Modulation type: pulsed
ばく露時間: intermittent 7 hours per day for 5, 14 or 20 days

ばく露1

主たる特性
周波数 50 Hz
タイプ
  • magnetic field
波形
  • pulsed
ばく露時間 intermittent 7 hours per day for 5, 14 or 20 days
Additional information (remark EMF-Portal: or sinusoidal signal? Contradictory specifications in the article)
Modulation
Modulation type pulsed
Pulse width 40 ms
Duty cycle 60 %
ばく露装置
ばく露の発生源／構造
チャンバの詳細 solenoid inside an incubator with 5% CO2 and 37°C
ばく露装置の詳細 samples were placed at the center of a uniform field area inside a multi-turn solenoid
パラメータ
測定量 値 種別 Method Mass 備考
磁束密度 1 mT - - - -

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露中
  • ばく露後

研究の主なアウトカム（著者による）

The gene expression of MAP2 was significantly increased in cells exposed to the magnetic field (group 1) for 14 days and in cells treated with neural differentiation medium (group 2), with group 2 showing the most distinct increase. However, no MAP2 expression was found in cells co-exposed to the magnetic field and differentiation medium (group 3). The Nestin gene expression was significantly increased in cells from group 1 after 14 days and group 3, with the most distinct increase in group 3.
In morphological investigations, cells from group 1 showed the unipolar and multipolar morphology of neurons after 5 or 14 days, while in group 2, a large number of dead cells was observed.
Cell proliferation was decreased in group 1 after 5, 14 and 20 days compared to the control group (significance not stated), supporting an increased differentiation in this group.
The authors conclude that exposure of dermal papilla mesenchymal cells to a pulsed 50 Hz magnetic field might increase the gene expression of the neural differentiation markers Nestin and MAP2.

研究の種別:

研究助成

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