この研究は、BALB/cマウスを用いて、卵母細胞の分化と卵胞発育に対する低周波磁界(50Hz、3 mT)ばく露の影響を調べた。ばく露群(n=15)、擬似ばく露群(n=15)は、妊娠の全期間、1日4時間の磁界ばく露(擬似ばく露)を受けた。両群から生まれた雌の新生仔の半分は出産時に、残りの半分は成獣に達した時点で、それぞれの卵母細胞を摘出して検査した。その結果、擬似ばく露群に比べ、ばく露群では、卵母細胞巣がほとんど破壊され、不規則に並んでいた;原始卵胞は生育が遅れ、電子顕微鏡像では卵母細胞の核の異質染色質と縮み、空胞化した細胞質が観察された、と報告している。
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To investigate the effects of extremely low frequency magnetic fields on the oocyte differentiation and follicular development in mice.
Pregnant mice were divided into an exposure and a sham exposure group (n=15 per group). Exposure/sham exposure took place during the pregnancy. After delivery, half of the female pups of both groups were sacrificed, while the other half was kept under normal conditions (no exposure) and sacrificed when they reached adulthood.
ばく露 | パラメータ |
---|---|
ばく露1:
50 Hz
ばく露時間:
4 hours/day during pregnancy (21 days)
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|
周波数 | 50 Hz |
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タイプ |
|
ばく露時間 | 4 hours/day during pregnancy (21 days) |
ばく露の発生源/構造 |
|
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Sham exposure | A sham exposure was conducted. |
測定量 | 値 | 種別 | Method | Mass | 備考 |
---|---|---|---|---|---|
磁束密度 | 3 mT | - | - | - | - |
Ovaries from exposed mice showed oocyte nests that were mostly broken and irregular arranged, while in the sham exposure group oocyte nests were intact and regularly arranged. Primordial follicles of mice from the exposure group were less developed than those from the sham exposure group and oocytes appeared to contain heterochromatin and were shrunken with vacuolated cytoplasm.
The authors conclude that extremely low frequency magnetic fields could influence the oocyte differentiation and follicular development in mice and that this may impair fertility.
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