研究のタイプ: 医学／生物学の研究 (experimental study)

[1950 MHzのUMTS無線周波電磁界にインビトロばく露したヒト白血球における遺伝毒性的影響の評価] med./bio.

Evaluation of genotoxic effects in human leukocytes after in vitro exposure to 1950 MHz UMTS radiofrequency field

著者: Zeni O, Schiavoni A, Perrotta A, Forigo D, Deplano M, Scarfi MR
掲載誌: Bioelectromagnetics 2008; 29 (3): 177-184
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この研究は、第3世代無線電話技術である1950MHzのUMTS無線周波電磁界（RF）にインビトロばく露したヒト白血球における遺伝毒性的影響を調べた。6人の健常者から末梢血を採取し、1950MHzのRFへの断続ばく露（6分間ばく露、2時間休止）を行った。インキュベータ内に置いたTEMセルを用いてばく露し、SARは2.2W/kgとした。さまざまな細胞分裂周期の段階を考慮して、24j時間から68時間までのばく露を行い、ばく露後、標準的な細胞毒性指標を検査した。その結果、異なる細胞分裂周期におけるばく露は小核細胞を増加させず、細胞分裂周期の動力学にも変化を起こさなかった；ばく露直後にDNA構造の変化は見られなかった、と報告している。

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研究目的（著者による）

To study whether intermittent exposures at the frequency of 1950 MHz and UMTS signal are able to induce genotoxic effects in human blood cells.

詳細情報

For each donor (n=6) several experimental conditions were considered to check for a possible different sensitivity through the cell cycle (via micronucleus assay): (1) intermittent exposures of un-stimulated lymphocytes (resting cells) for 24 h, followed by 44 h exposure after phytohemagglutinin stimulation, to test radiofrequency exposure in G0 phase-G1 phase-S phase-G2 phase stages; (2) intermittent exposures for 44 h after phytohemagglutinin stimulation, to test radiofrequency exposure in G1 phase-S phase-G2 phase stages; (3) intermittent exposures of un-stimulated lymphocytes (resting cells) for 24 h before phytohemagglutinin stimulation. For comet assay un-stimulated lymphocytes (resting cells) were exposed for 24 h.
Positive controls were performed by treating cells with mitomycin C and methyl methanesulfonate for micronucleus and comet assays, respectively.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 1,950 MHz
ばく露時間: intermittent, 6 min on/2 h off, for 24, 44, or 24 + 44 h
  • SAR: 2.2 W/kg

ばく露1

主たる特性
周波数 1,950 MHz
タイプ
  • electromagnetic field
特性
  • guided field
ばく露時間 intermittent, 6 min on/2 h off, for 24, 44, or 24 + 44 h
Modulation
Modulation type cf. additional information
Additional information

UMTS 3GPP standard (FDD mode)
ばく露装置
ばく露の発生源／構造
チャンバの詳細 The TEM cell was placed vertically inside an incubator equipped with air circulation systems.
ばく露装置の詳細 Two 25-ml Pyrex flasks filled with 10 ml of whole blood culture were placed 1 cm from each other on each side of the septum over a 1.5-mm thick Plexiglas shelf and radiated from the bottom.
Sham exposure A sham exposure was conducted.
Additional information Several independent temperature measurements were carried out in dummy cultures (flasks filled with culture medium) applying constant input power of 6 W for 6 min. A temperature increase of 0.67 ± 0.35 °C was measured, but the "off" period (2 h) was long enough to restore the initial conditions.
パラメータ
測定量 値 種別 Method Mass 備考
SAR 2.2 W/kg - 計算値 - -
測定および計算の詳細

Power was checked at different points of the feeding line throughout the exposure. The electric field and SAR distributions were determined using FDTD computational dosimetry considering four different materials. The electromagnetic characteristics for glass, plastic material, and blood were obtained from literature, while culture permittivity and conductivity were measured.

Reference articles

  • Zeni O et al. (2003): [900 MHz電磁界にばく露されたヒトリンパ球には遺伝毒性（微小核誘導）が見られない]

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

The data showed that intermittent exposures in different stages of the cell cycle to the UMTS signal at SAR value of the current European safety limit do not induce genotoxic or cytotoxic effects in human lymphocytes.

研究の種別:

研究助成

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