この研究は、1.8GHz無線周波放射(RFR)と突然変異原である4種の化学物質の併用でヒトのリンパ球に誘発されるDNA損傷の相乗効果の有無を調べた。一人の健常男性から採取した血液から分離、培養したリンパ球で、(1) 擬似ばく露群、(2) RFRばく露群、(3) 化学物質ばく露群、(4) RFR + 各化学物質(ばく露順は3通り)を用意した。組合せばく露の順序は、(1) RFR、各化学物質の順、 (2) 同時ばく露、(3) 各化学物質、RFRの順、である。4種の化学物質は、ミトマイシンC(MMC)、ブレオマイシン(BLM)、メチルメタンスルホン酸塩(MMS)、ニトロチン(4NQO)である。各物質とも4通りの投与濃度で、それぞれ3時間培養した。RFばく露には、2つのR18方形導波管チャンバを用意し、1.8 GHzのGSM信号へのばく露または擬似ばく露をブラインド条件で2時間行った。SARは3 W/kg、温度は37±0.08113 ?Cであった。RFR(2時間ばく露)および/または化学物質(3時間ばく露)の全ての処置終了後、0時間培養および21時間培養したものをコメットアッセイし、テール長(TL)とテールモーメント(TM)を指標にDAN損傷を検査した。その結果、0および21時間培養のどちらでも、RFR群と対照群でDNA損傷に有意差はなかった;同様にどちらの培養でも、MMC群とRFR+MMC組合せばく露群の間、ならびに4NQOg群とRFR+4NQO組合せばく露群の間には、DNA損傷の有意差が見られた(組合せばく露の順序のいずれにおいても);BLMおよびMMSでは、RFRばく露による損傷増加は見られなかった、と報告している。
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To study the synergistic DNA damage effects in human lymphocytes induced by 1.8 GHz radiofrequency field irradiation with four chemical mutagens (mitomycin C (DNA crosslinker), bleomycin (radiomimetic agent), methyl methanesulfonate (alkylating agent), and 4-nitroquinoline-1-oxide (UV-mimetic agent)).
Cells were divided into four groups: 1) sham-control group, 2) radiofrequency field irradiation group, 3) chemical exposure group, 4) radiofrequency field irradiation + chemical exposure group. Three combinative exposure ways were: 1) radiofrequency field irradiation before chemical exposure, 2) radiofrequency field irradiation together with chemical exposure, and 3) radiofrequency field irradiation after chemical exposure.
ばく露 | パラメータ |
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ばく露1:
1.8 GHz
ばく露時間:
continuous for 2 h
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|
周波数 | 1.8 GHz |
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タイプ |
|
特性 |
|
ばく露時間 | continuous for 2 h |
Modulation type | unspecified |
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ばく露の発生源/構造 | |
---|---|
チャンバの詳細 | The exposure setup was based on two R18 rectangular waveguide chambers, one for RF and one for sham exposure that were blindly activated by a computer controlled signal unit. |
ばく露装置の詳細 | Cells were put in 35-mm dishes and placed into the two waveguide chambers (TEM cells). |
Sham exposure | A sham exposure was conducted. |
Additional information | The lymphocytes were divided into four groups: sham control, RF exposure, chemical exposure, and RF + chemical exposure group. |
測定量 | 値 | 種別 | Method | Mass | 備考 |
---|---|---|---|---|---|
SAR | 3 W/kg | unspecified | 指定なし | - | - |
The data showed no difference of DNA damage indices between radiofrequency field exposed group and control group at 0 and 21 h incubation after exposure.
There were significant differences of DNA damage indices between "mitomycin C group" and "radiofrequency field irradiation + mitomycin C co-exposure group" at 0 and 21 h incubation after treatment.
Also the significant difference of DNA damage indices between "4-nitroquinoline-1-oxide group" and "radiofrequency field irradiation + 4-nitroquinoline-1-oxide co-exposure group" at 0 and 21 h incubation after treatment was revealed.
The DNA damage in "radiofrequency field irradiation + bleomycin co-exposure groups" and "radiofrequency field irradiation + methyl methanesulfonate co-exposure groups" was not significantly increased, as compared with corresponding "bleomycin" and "methyl methanesulfonate groups".
The findings indicated that 1.8 GHz radiofrequency field irradiation for 2 h did not induce the human lymphocyte DNA damage effects in vitro, but it could enhance the human lymphocyte DNA damage effects induced by mitomycin C and 4-nitroquinoline-1-oxide. The synergistic DNA damage effects of 1.8 GHz radiofrequency field irradiation with bleomycin or methyl methanesulfonate were not obvious.
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