研究のタイプ: 医学／生物学の研究 (experimental study)

[900 MHz放射は異なる種類の細胞に小核形成を誘導しない] med./bio.

900 MHz radiation does not induce micronucleus formation in different cell types

著者: Hintzsche H, Jastrow C, Kleine-Ostmann T, Schrader T, Stopper H
掲載誌: Mutagenesis 2012; 27 (4): 477-483
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この研究は、ゲノム損傷の現れとしての有糸分裂擾乱の結果生じる可能性がある小核の誘導を指標に、900MHzの携帯電話電磁界の影響を、ヒト細胞としてHaCat細胞、ヒト‐ハムスター・ハイブリッド細胞としてA(L)細胞の2種類の細胞を用いて調べた。ばく露後の異なるインキュベーション期間の後、細胞を固定し、小核の発生頻度を評価した結果、どちらの種類の細胞にもばく露によるゲノム損傷は見られなかったと報告している。

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研究目的（著者による）

To study the potential of radiofrequency electromagnetic fields (900 MHz) to cause a genomic damage in the form of micronucleus induction.

詳細情報

After different post-exposure incubation periods (4 h or 22 h, with or without cytochalasin B), cells were fixed and micronucleus frequencies were evaluated. Cells treated with methyl methanesulfonate for 4 h were used as positive control.

影響評価項目

ばく露

ばく露 パラメータ
ばく露1: 900 MHz
Modulation type: CW
ばく露時間: continuous for 30 min (both cell types) or 22 h (AL cells only)
both cell types
ばく露2: 900 MHz
Modulation type: pulsed
ばく露時間: continuous for 30 min
AL cells only

ばく露1

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • far field
ばく露時間 continuous for 30 min (both cell types) or 22 h (AL cells only)
Additional information both cell types
Modulation
Modulation type CW
ばく露装置
ばく露の発生源／構造
ばく露装置の詳細 cells placed in slide flasks positioned in the center of the µTEM cell; a µTEM cell is a widened coaxial waveguide in which the inner conductor transforms from a round wire at the input and output ports to a thin plate in the center; above and beneath this plate the field is homogeneous; slide flasks at the bottom of the housing underneath the plate; µTEM cell temperature-regulated with a Peltier element and placed inside a thermally isolating styrofoam box; µTEM cell terminated with a 50 Ω resistor to ensure travelling wave conditions
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
電界強度 90 V/m effective value 計算値 - 5, 10, 30, 90 V/m

ばく露2

主たる特性
周波数 900 MHz
タイプ
  • electromagnetic field
特性
  • far field
ばく露時間 continuous for 30 min
Additional information AL cells only
Modulation
Modulation type pulsed
Additional information

GSM - all eight time slots set to normal burst (GMSK/full rate)
ばく露装置
ばく露の発生源／構造
  • E1と同じ装置
Sham exposure A sham exposure was conducted.
パラメータ
測定量 値 種別 Method Mass 備考
電界強度 90 V/m effective value 計算値 - 5, 10, 30, 90 V/m

ばく露を受けた生物:

方法 影響評価項目／測定パラメータ／方法

研究対象とした生物試料:
調査の時期:
  • ばく露後

研究の主なアウトカム（著者による）

Both cell types did not show any genomic damage after exposure. To adapt the protocol for the micronucleus test into the direction of the protocol for mitotic disturbances, the post-exposure incubation period was reduced (4 h). and exposure time was extended to one cell cycle length (22 h). This did not result in any increase of the genomic damage.
In conclusion, micronucleus induction was not observed as a consequence of exposure to non-ionizing radiation, even though this agent was reported to cause mitotic disturbances under similar experimental conditions (see Schmid and Schrader 2007, Schrader et al. 2008, Schrader et al. 2011).

研究の種別:

研究助成

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