この研究は、仔ラットの小脳に対する出生前の電磁界ばく露の影響を調べた。妊娠が判明したWister雌ラットをばく露群(n=10)、対照群(n=10)に分け、ばく露群は妊娠第一日目から出産日まで、1日6時間、市販の携帯電話の900MHzパルス電磁界にばく露した。電話機とプラスチックケージとの距離は40cm、SARは0.5 - 0.9 W/kgと述べている。両群から出生した仔ラットのうち、各群10匹について、出生後30-32日目に種々の行動テスト(運動学習および小脳機能に関するタスク)と電気生理学的評価(小脳のプルキンエ細胞標本の電気的活動のホールセルパッチクランプ法による測定)を行った。その結果、ばく露群において行動の異常は何も見られなかった;その一方、ばく露群のプルキンエ細胞に興奮性低下が見られた;特に、後過分極振幅、スパイク頻度、半値幅、第1スパイク遅延に大きな変化が見られた、と報告している。
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To assess whether maternal exposure to 900 MHz electromagnetic fields would adversely affect the cerebellar function of rat offspring using electrophysiological and behavioral studies.
Pregnant rats were divided into a control group (n=10) or a mobile phone exposure group (n=10). Pups were weaned on postnatal day 23 and all experiments were performed on postnatal days 30-32.
周波数 | 900 MHz |
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タイプ |
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ばく露時間 | 6 h/day during whole gestation |
Modulation type | pulsed |
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ばく露の発生源/構造 |
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チャンバの詳細 | Plexiglas cages |
ばく露装置の詳細 | a cell phone and Plexiglas cage with the animals were placed inside a Faraday cage; the Plexiglas cage was placed 40 cm away from the cell phone |
Sham exposure | A sham exposure was conducted. |
The data did not show any behavioral changes in rats chronically exposed to electromagnetic fields. However, patch-clamp recordings revealed a decreased neuronal excitability of Purkinje cells in exposed rats. The most significant changes were observed when recording the spontaneous activity of neuronal excitability including the after-hyperpolarization amplitude, spike frequency and half width.
Pregnancy length, body temperature and litter sizes of exposed dams were not significantly different from values of sham exposed dams. Mobile phone exposure did not significantly affect mortality rate and the body weights of pups.
In conclusion, the findings showed that prenatal exposure to electromagnetic fields resulted in altered electrophysiological properties of Purkinje cells. Both male and female pups were sensitive to many electrophysiological effects following maternal exposure. However, these changes may not be severe enough to alter the cerebellum-dependent functionality.
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